@article {1355, title = {Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {162-172}, type = {Original Article}, chapter = {162}, abstract = {

Background: The abstract provides a comprehensive overview of the study on secondary metabolites from Ipomoea sagittifolia, emphasizing the extraction, isolation and characterization processes. Secondary metabolites, including alkaloids, flavonoids, phenols and glycosides, were identified and analyzed using various analytical techniques. Materials and Methods: Ethanol extraction followed by Soxhlet apparatus yielded a crude extract, subsequently purified through column chromatography using a solvent mixture of n-butanol, glacial acetic acid and water. Thin Layer Chromatography (TLC) confirmed the presence of compounds across 108 eluted fractions, further validated by UV-vis spectroscopy revealing absorption peaks characteristic of glycosides at 288 nm. Results: Fourier Transform Infrared (FTIR) spectroscopy highlighted functional groups such as alcohols, phenols, carboxylic acids and ketones, typical of glycoside structures. High-Performance Liquid Chromatography (HPLC) confirmed the presence of cardiac glycosides through retention time analysis, aligning closely with Scillaren A standards. Conclusion: These findings underscore the pharmacological potential of Ipomoea sagittifolia in traditional medicine and pharmaceutical applications, supported by its diverse secondary metabolite profile. Future research could delve deeper into bioactivity assays to elucidate specific therapeutic properties linked to these metabolites, thereby enhancing its relevance in healthcare and biotechnological innovations.

}, keywords = {Bioactive, Elucidation, High performance thin layer chromatography, Ipomea}, doi = {10.5530/pres.20251964}, author = {Raveesha Peeriga and Krishnaveni Manubolu and Jayaprakash Somasundaram and Sheikdawood Asanar Mohamed Shiek Arabath and Shanthi Petchimuthu and Sri Naga Joshitha Manepalli and Vinay Kumar Maddela and Kavya Makireddy} } @article {778, title = {Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {May,2014}, pages = {234-239}, type = {Original Article}, chapter = {234}, abstract = {

Background: Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential. Objective: The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves{\textquoteright} ethanolic extract by analytical techniques. Materials and Methods: Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening. Results: Extraction yield was found to be 13.94\% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60\% w/w and 4.03\% w/v, respectively. Conclusion: The active constituent isolated was found to be equal to rutin.

}, keywords = {High performance thin layer chromatography, High-pressure liquid chromatography, Polyalthia longifolia, Rutin, Thin layer chromatography}, doi = {10.4103/0974-8490.132601}, author = {Gaurav Mahesh Doshi and Sandeep Prabhakar Zine and Pratip Kashinath Chaskar and Hemant Devidas Une} }