@article {1449, title = {Phytochemical Profiling, Antioxidant and Antimicrobial Activities of a Traditional Polyherbal Formulation Containing Citrus limon, Bergera koenigii, Citrus medica and Andrographis paniculata}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {881-895}, type = {Original Article}, chapter = {881}, abstract = {

Background: Polyherbal formulations combining medicinal plants are widely studied for their enhanced therapeutic potential. Materials and Methods: This study evaluates a polyherbal formulation containing leaves of Citrus limon, Bergera koenigii, Citrus medica, and Andrographis paniculata for its antimicrobic and antioxidative properties. An ethanolic extract was examined to determine its phytochemical composition, which revealed a rich existence of bioactive substances like bioflavonoids, alkali-like compounds, phenolics, and Steroidal glycoalkaloids. Results: In vitro Total Antioxidant Capacity (TAC), including DPPH radical, FRAP and scavenging of nitric oxide, exhibited significant radical-neutralizing capacities comparable to standard antioxidants. The formulation also exhibited potent antibacterial action against strains of fungi and bacteria, both gram-positive and gram-negative, suggesting a synergistic enhancement of efficacy due to the combination of plant extracts. Conclusion: The outcomes highlight the prospect of this polyherbal extract as a natural therapeutic agent to treat microbial infections and oxidative stress. The future studies should focus upon in vivo evaluations and elucidating molecular mechanisms to validate these promising results.

}, keywords = {Natural therapeutics, Oxidative stress, Phytochemicals, Polyherbal formulation}, doi = {10.5530/pres.20252131}, author = {Muthuswamy Gnanadeepan and Kunnathur Murugesan Sakthivel and Rajan Radha Rasmi and Venkatraman Thirumalai and Kumar Pavithra and Kannikaparameswari Nachimuthu} } @article {1377, title = {A Unique Method for Simultaneous HPLC Analysis of Gallic Acid, Glycyrrhizic Acid and E-Guggulsterone in a Polyherbal Formulation}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {295-306}, type = {Original Article}, chapter = {295}, abstract = {

Background: The therapeutic effects of herbal medicines are exerted due to the presence of different phytoconstituents and the effects are further potentiated when compatible herbals are formulated together. High Performance Liquid Chromatography (HPLC) is an important analytical tool towards standardization of polyherbal formulation. Complex nature, co- existence with other constituents trace quantities of active phytoconstituents and their simultaneous estimation are challenges encountered in HPLC analysis of polyherbal formulation. Simultaneous estimation of active biomarkers in polyherbal formulation by HPLC provides a unique method for verifying the quality and efficacy of polyherbal formulation. Materials and Methods: Present research work is about inventing an unique, selective and sensitive HPLC (High Performance Liquid Chromatography) method for simultaneous estimation of Gallic acid, Glycyrrhizic acid and E-Guggulsterone from a polyherbal formulation containing thirteen Ethnobotanical herbs, reported to have Cardioprotective effects, marketed as Collasyn tablets by Dr. Palep Research Labs Ltd. in Indian market. Reverse-phase column (C18, 250 mM X4.6 mM i.d., 5μm) was used to analyze the marker compounds Gallic acid, Glycyrrhizic acid and E-Guggulsterone at λmax 224 nm. The mobile phase was composed of acetonitrile and 0.05\% OPA (Ortho-phosphoric acid) in water (50:50) with a flow rate of 1 mL/min. Results: A unique HPLC method is developed for simultaneous estimation of active markers-Gallic acid, Glycyrrhizic acid and E-Guggulsterone from the polyherbal formulation. Quantitative analysis shows Gallic acid, Glycyrrhizic acid and E-Guggulsterone to be 11.47\%, 1.05\% and 0.63\% respectively in the polyherbal formulation. The developed method is validated in accordance with the International Council for Harmonisation of Technical Requirements for Pharmaceuticals guidelines. The method shows good linearity in a relatively wide range of Concentration of 50-150 mcg/mL. The method is also found to be precise, accurate and robust. Conclusion: This unique and validated method for simultaneous estimation of cardioprotective biomarkers Gallic acid, Glycyrrhizic acid and E-Guggulsterone from polyherbal formulation, marketed as Collasyn tablets by Dr. Palep Research Labs Ltd. is important for its quality control. It helps in harnessing the complete plant potential for therapeutics. Thus, this approach to standardizing polyherbal formulations will be instrumental in verifying the quality and efficacy of the polyherbal extract. The simultaneous estimation of constituents from plants presents a significant advancement in phytochemical analysis, offering a comprehensive understanding of plant properties and their potential applications. The methodologies employed demonstrate high efficiency and accuracy, allowing for the effective quantification of multiple compounds in a single analytical run. This approach not only streamlines the process of phytochemical profiling but also enhances the ability to explore the therapeutic potentials of various plant species.

}, keywords = {Cardioprotective, HPLC, Method development, Method validation, Polyherbal formulation}, doi = {10.5530/pres.20252013}, author = {Bharati Gawade and Swati Patil} } @article {1263, title = {Standardization of Madhumukthi Kudineer Chooranum Using Pharmacognostic, Physicochemical and HPTLC Studies}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {531-539}, type = {Original Article}, chapter = {531}, abstract = {

Background: Siddha system of medicine is considered to be foremost among other systems of medicine. Many medicines are available in traditional systems for all kind of diseases. Most people show their interest towards Siddha medicine for non-communicable diseases especially for diabetes, hypertension, etc., Poly herbal formulations like Avarai kudineer has been authorized for prescription for Madhumegam. Madhumukthi Kudineer Chooranum (MKC) is one such medicine which has been found efficacious in Madhumegam (Diabetes mellitus). The current study is aimed at standardizing MKC employing pharmacognostic and chemical analyses with the aim of developing standards for quality control of this important medicine of Siddha. Materials and Methods: MKC was analyzed for pharmacognostic, physiochemical and HPTLC standards following standard procedures mentioned in Pharmacopoeias. Results: Macroscopy, powder microscopy was matched with the characters of these ingredients as reported in pharmacopoeias. The physicochemical standards were correlated with standards. TLC of MKC showed 10 spots under UV 254 nm, 13 spots under UV 366 nm and after spray with 5\% vanillin sulphuric acid solution. Conclusion: The reported results will be supportive for standardization and future studies of MKC.

}, keywords = {Antidiabetic, Antioxidant, Kudineer chooranum, Madhumegam, Polyherbal formulation}, doi = {10.5530/pres.16.3.63}, author = {Rajamanickam Baskar and Govindaraju Bharathkumar and Koppala Narayana Sunil Kumar and Ramachandran Shakila and Manekshah Yovas Rajammal and Sikkal Selvaraaju Selvapriya and Thangarasu Hema Devi} } @article {1082, title = {Development of a Gastroretentive Polyherbal Formulation and its Standardization}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {October 2022}, pages = {379-390}, type = {Original Article}, chapter = {379}, abstract = {

Background: Phytopharmaceuticals within the shape of the entire herb, plant parts, or separated bioactive constituents have been utilized broadly in therapeutics since antiquated times till date. The appealing use of drugs of the natural root has driven the requirement for standardization as per the WHO guidelines. Objectives: The main aim of the existing study was to formulate polyherbal gastroretentive capsules by using Carbopol 934 and 971P in different concentration ranges to get desired buoyancy rate. Materials and Methods: The prepared formulation was characterized by physico-chemical parameters and pharmaceutical quality control tests. Standard procedures were used to estimate the organoleptic properties, extractive values, and ash values of the crude drugs used in formulations. Extraction was carried out using Soxhlation followed by preliminary phytochemical evaluation and HPTLC fingerprinting of the extracts. The extracts were formulated into a gastroretentive polyherbal capsule and assessed for physical parameters. Results: The phytochemical assessment established the presence of flavonoids, alkaloids, saponins, steroids, and glycosides. Rf values obtained from the HPTLC studies of the extracts signified the presence of important phytoconstituents such as Shogaols, 6-Gingerol, 8-Gingerol, Glycyrrhizin, Andrographolides, Marmelosin, and Conessine. Percent Carr{\textquoteright}s consolidation index ranging from 8.111 {\textpm} 0.157 to 8.827 {\textpm} 0.186, Hausner{\textquoteright}s ratio between 1.088 {\textpm} 0.002 to 1.097 {\textpm} 0.002, and angle of repose below 30 are indicative of excellent flow property of the granules. Conclusion: The gastroretentive capsules PHF4 containing granules formulated using 75 mg Carbopol 971P showed a buoyancy effect of more than 24 hr with desired structural integrity.

}, keywords = {Extraction, HPTLC fingerprinting, Polyherbal formulation, Standardization}, doi = {10.5530/pres.14.4.56}, author = {Manish Kumar Gupta and Medha Amol Khade and Birendra Srivastava and Supriya Rajesh Hyam and Prashant Basappa Gurav} } @article {759, title = {Standardization of some herbal antidiabetic drugs in polyherbal formulation}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {49-56}, type = {Original Article}, chapter = {49}, abstract = {

Background:\ Ayurvedic formulations are used to treat a wide variety of diseases including diabetes mellitus Standardization of herbal formulation is essential in order to assess the quality of drugs. The present paper reports standardization of eight herbal anti-diabetic drugs-Momordica charantia\ (seeds),\ Syzigium cumini\ (seeds),\ Trigonella foenum\ (seeds),\ Azadirachta indica\ (leaves),\ Emblica officinalis\ (fruits),\ Curcuma longa\ (rhizomes),\ Gymnema sylvestre\ (leaves),\ Pterocarpus marsupium\ (heart-wood) individually and in polyherbal marketed samples of Baidyanath Madhumehari Churna\ Material and Methods:\ Shivayu Madhuhari Churna, Meghdut Madhushoonya Churna and were compared to the in-house preparation for physicochemical properties.\ Results and Conclusions:\ The limits obtained from the different physicochemical parameters of the individual eight herbal drugs and the marketed formulations could be used as reference standard for standardization of the anti-diabetic drugs in a quality control laboratory.

}, keywords = {Herbal antidiabetic drugs, Physicochemical parameters, Polyherbal formulation, Standardization}, doi = {10.4103/0974-8490.79116}, author = {Harinarayan Singh Chandel and AK Pathak and Mukul Tailang} } @article {797, title = {Standardization of Ajmodadi churna, a Polyherbal Formulation}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {May 2010}, pages = {98-101}, type = {Original Article}, chapter = {98}, abstract = {

Standardization of herbal formulations is essential in order to assess the quality of drugs, based on the concentration of their active principles. This article reports on standardization of Ajmodadi churna, a polyherbal ayurvedic medicine used as a carminative and an antispasmodic, and is a strong wormifuge, and helps in all painful conditions like sciatica and stiffness in back and also restores normal digestive functions. Ajmodadi churna was prepared as per Ayurvedic Formulary of India. In-house preparation and the marketed drug have been standardized on the basis of organoleptic characters, physical characteristics, and physico-chemical properties. The set parameters were found to be sufficient to evaluate the churna and can be used as reference standards for the quality control/quality assurance laboratory of a Pharmaceutical house.

}, keywords = {Ajmodadi churna, Physico-chemical, Polyherbal formulation, Standardization}, doi = {10.4103/0974-8490.62957}, author = {Neeraj K Sriwastava and CS Shreedhara and HN Aswatha Ram} } @article {829, title = {Standardisation of Avipattikar Churna- A Polyherbal Formulation}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {224-227}, type = {Original Article}, chapter = {224}, abstract = {

Standardisation of herbal formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. The present paper reports on standardisation of Avipattikar churna, a poly herbal ayurvedic medicine used as remedy for acidity and complications associated with it like headache, nausea and vomiting. It is also used as laxative and helps to increase appetite. Avipattikar churna was prepared as per Ayurvedic Formulary of India. In-house preparation and two marketed have been standardised on the basis of organoleptic characters, physical characteristics and physico-chemical properties. The set parameters were found to be sufficient to evaluate the churna and can be used as reference standards for the quality control/quality assurance laboratory of a Pharmaceutical house.

}, keywords = {Avipattikar churna, Physicochemical parameters, Polyherbal formulation, Standardisation}, doi = {Nil}, author = {Aswatha Ram HN and Kaushik Ujjwal and Lachake Prachiti and Shreedhara CS} }