@article {1281, title = {Screening of in vitro Anticancer Activity of Various Human Cancer Cell Lines and Induced Apoptosis and Cell Arrest by Ethanolic Bulb Extract of Pancratium zeylanicum}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {718-726}, type = {Original Article}, chapter = {1}, abstract = {

Aim: World widely the human society suffers from various types of cancer due to many causes. Thus, finding out a good anticancer agent is a need of the hour. Medicinal herbs are always considered at pole position whenever we discuss anticancer properties. Numerous herbal plants have been assessed and are subjected to phytochemical analysis to learn more about their anticancer effects. Materials and Methods: To assess the anti-cancerous activity of PZBE extract in MTT assay, cell cycle analysis, and apoptosis studies have been performed using different cancer cell lines. Results: This study aims to assess the impact of ethanolic extract of Pancratium zeylanicum Bulbs (PZBE) against cancerous cells in vitro conditions. MTT assay was used to compare the PZBE extract with Annexin V as a positive control compound against four human cancer cell lines, including Human cervical cancer (HeLa), colon cancer (HT-29), lung cancer (A549) and breast cancer (MCF-7) cell lines with the IC50 values of 209.28 μg/mL for HeLa, 133.09 μg/mL for A-549, 264.46 μg/mL for MCF-7 and 264.75 μg/mL for HT-29 cell lines. Increasing of the populations observed in G2-M and G0-G1 cells by 42\% and 44.78\%, respectively when compared to the control. The graph demonstrates that PZBE extracts significantly induced G0-G1 arrest in A-549 cells. Furthermore, the PZBE extract effectively induced apoptosis when compared to untreated cells, A-549 cells treated with IC50 concentration, and annexin-V double-staining assay demonstrated an increase in the percentage of early and late apoptotic cells. Conclusion: The ethanol extract of Pancratium zeylanicum bulbs demonstrated potent cytotoxic activity against the cancer cell lines HeLa, A-549, MCF-7, and HT-29. The current study{\textquoteright}s findings may be helpful in the development of plant-based anticancer drugs.

}, keywords = {A-549, Annexin-V, Anticancer activity, Apoptosis, Ethanol extract, HeLa, HT-29, MCF-7}, doi = {10.5530/pres.16.4.83}, author = {Elumalai Shanthi Priya and Devaraj Sathiya Sheela and Devaraj Sheeba Gnanadeebam and Balrajmani Davidson Raja and Periannan Viswanathan} } @article {717, title = {In vitro antiproliferative activity of Annona reticulata roots on human cancer cell lines}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {09-12}, type = {Original Article}, chapter = {09}, abstract = {

Background:\ The phytochemical and pharmacological activities of\ Annona reticulata\ components suggest a wide range of clinical application in lieu of cancer chemotherapy.\ Materials and Methods:\ Ethanol and aqueous extracts of roots of\ Annona reticulata\ Linn were studied for their\ in vitro\ antiproliferative activity on\ A-549\ (human lung carcinoma),\ K-562\ (human chronic myelogenous leukemia bone marrow), HeLa\ (human cervix) and\ MDA-MB\ (human adenocarcinoma mammary gland) cancer cell lines by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.\ Results:\ The ethanol extract exhibited a prominent inhibitory effect against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines at a concentration range between 10 and 40 μg/ml, whereas the aqueous extract showed a lower activity at the same concentration. Simultaneously, the effect of the ethanol extract toward the inhibition of\ Vero\ cell line proliferation was lower in comparison with the cancer cell lines.\ Conclusion:\ The significant antiproliferative activity of the ethanol extract of\ Annona reticulata\ roots against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines may be attributed toward the collective presence of acetogenins, alkaloids and lower inhibitory effect on\ Vero\ cell line, which suggests\ Annona reticulata\ be used as a chemopreventive agent in cancer therapy.

}, keywords = {A-549, Annona reticulata, Antiproliferative activity, HeLaand MDA-MBhuman cancer cell lines, K-562, MTT assay}, doi = {10.4103/0974-8490.79109}, author = {HM Suresh and B Shivakumar and K Hemalatha and SS Heroor and DS Hugar and K.R.S Sambasiva Rao} }