@article {1181, title = {Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {727-732}, type = {Original Article}, chapter = {727}, abstract = {

Background: A number of nonsteroidal anti-inflammatory medications have been shown to lessen pain and inflammation. Unfortunately, there are numerous side effects connected with their use. There are, however, medicinal plants that have anti-inflammatory therapeutic effects with little to no side effects. Momordica dioica is a herb that belongs to the Cucurbitaceae family. There are numerous phytoconstituents in it. Ursolic acid is one of them and a pentacyclic terpenoid that is found in nature and has a number of therapeutic benefits. The current study looked at Ursolic Acid{\textquoteright}s activities (UA), a secondary plant metabolite, for its anti-inflammatory properties in the Momordica dioica plant. The current study{\textquoteright}s objective was to create and validate an HPTLC method that was quick, accurate, precise, and specific for determining ursolic acid from Momordica dioica herbal extract. Methodology/Conclusion/Significance: For quick analysis of Ursolic acid determination, The High Performance Thin Layer Chromatography (HPTLC) was established and confirmed. On an aluminium HPTLC plate (60 F254) coated with precoated silica gel with formic acid, ethyl acetate, and toluene (7:3:0,1), chromatographic separation was accomplished. The detection process was carried out at 530 nm. Ursolic acid{\textquoteright}s Rf value was discovered to be 0.795\%. In the 400ng/band concentration range, linearity for ursolic acid was detected. The limits of detection and quantitation for ursolic acid were observed to be 0.04 ng/ band and 0.14 ng/band, respectively. The mean \% recovery of rosmarinic acid was (0.54). The method{\textquoteright}s specificity, robustness, linearity, precision, and accuracy have all been validated in compliance with ICH standards. The created method can be used to evaluate the regularity of ursolic acid in herbal extracts.

}, keywords = {Chromatography, Herbal extract, HPTLC, Inflammation, Methanol, momordica dioica, Rf value, Ursolic acid}, doi = {10.5530/pres.15.4.077}, author = {Jui Darbhe and Vrushali Neve and Vrushali Bhalchim and Ashwini Tonpe and Pritam Khandave and Suchita Mane and Rupali Yewale} } @article {705, title = {Effect of Momordica Dioica Fruit Extract on Antioxidant Status in Liver, Kidney, Pancreas, and Serum of Diabetic Rats}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {December,2013}, pages = {73-79}, type = {Original Article}, chapter = {73}, abstract = {

Background: Fruits, leaves, and tuberous roots of Momordica dioica are used as a folk remedy for diabetes mellitus (DM) in India. The aqueous extract of Momordica dioica fruit possesses very good anti-diabetic activity and is having high margin of safety. Objectives: The aim of the present study was to investigate the antioxidative effect of Momordica dioica fruits in alloxan-induced diabetic Wistar rats. Materials and Methods: Effect of aqueous extract of Momordica dioica (AEMD) on thiobarbituric acid reactive substances (TBARS), hydroperoxide (HP), non-enzymatic and enzymatic antioxidants in liver, kidney, pancreas, and serum was evaluated in diabetic rats after 21 days treatment. Results: Increase in the levels of TBARS, HP and decrease in the levels of non-enzymatic antioxidants and activity of enzymatic antioxidants was observed in liver, kidney, pancreas, and serum of diabetic rats when compared with normal healthy rats. TBARS and HP levels were reduced while non-enzymatic and enzymatic antioxidant enzymes activity was increased in AEMD and glibenclamide-treated rats. Furthermore, histological examination of liver, kidney, and pancreas of diabetic rats showed degenerative changes. AEMD treatment for 21 days rejuvenated liver, kidney, and pancreas histoarchitecture. Conclusion: In conclusion, the present results showed the protective role of AEMD on liver, kidney, and pancreas in severe diabetic rats justifying support for its anti-diabetic use in folk medicine.

}, keywords = {Alloxan, momordica dioica, Oxidative stress, Wistar rat}, doi = {10.4103/0974-8490.122922}, author = {Poonam Sharma and Rambir Singh} }