@article {995, title = {GC-MS analysis of Phytocomponents in the Methanol Extract of Premna latifolia Roxb}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {19-23}, type = {Original Article}, chapter = {19}, abstract = {

Background: According to existing literature, the genus Premna has a variety of biologically active secondary metabolites, but there are few findings on phytochemical screening of P. latifolia Roxb. Methanolic leaves extract. Objectives: Phytochemical screening of methanolic leaves extract of P. latifolia was proposed for this study. Materials and Methods: UV-chamber, HPTLC instrument (CAMAG TLC Scanner), and GC-MS instrument (Perkin-Elmer GC- Clarus) were used to analyse methanolic leaves extract of P. latifolia. To compare the peaks of components on chromatograms, the NIST library was employed. Results: The current study used GC-MS to identify probable chemical components of P. latifolia. The GC-MS analysis and NIST library comparison revealed that the methanolic extract of P. latifolia contained mainly Squalene (13.57 percent), Ergosta-5, 7, 9 (11), 22-tetraen-3-ol, (3. beta, 22E)-(0.15 percent), Stigmasterol (3.73 percent), gamma-Sitosterol (10.13 percent), Lupeol (0.33), beta-Amyrin (2.27 percent), alpha-Amyrin (2.05 percent), gamma-Sitostenone (0.35 percent), Ursolic aldehyde (1.01 percent) and Betulin (0.72 percent). The biological actions of the majority of the identified components have been reported. While scientific evidence of gamma-sitostenone{\textquoteright}s biological function is still not available. Conclusion: According to the findings, P. latifolia contains a variety of biologically active components, the majority of which are tri-terpenoids and phytosterols.

}, keywords = {GC-MS Analysis, HPTLC, Methanolic leaves extract., Phytocomponents TLC, Premna latifolia}, doi = {10.5530/pres.14.1.4}, url = {https://phcogres.com/fulltext/v14i1/105530pres1414.html}, author = {Rajesh Kumar and Brijesh Kumar and Ashutosh Kumar and Ajay Kumar and Manish Singh} } @article {987, title = {Phytochemical and GC-MS Analysis of Hydro Ethanolic Leaf Extract of Ocimum sanctum (L.)}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {October 2021}, pages = {233-237}, type = {Original Article}, chapter = {233}, abstract = {

Background: The pharmacological efficiency of herbal drugs has shown to be effective as conventional pharmaceutical drugs. Preliminary screening of herbal extracts helps in analysing the bioactive compounds present. Ocimum sanctum, the queen of herbs keeps a spiritual importance in Indian culture and have important place in traditional medicinal system of India. Objectives: The present study was implement to investigate the preliminary phytochemical screening and GC-MS analysis of leaf extract of O. sanctum to determine the phytoconstituents. Materials and Methods: Leaves collected were dried, crushed and mixed with ethanol and water and further applied for extract preparation. The prepare extract was used for phytochemical and GC-MS analysis. Results: In qualitative phytochemical analysis tannins, carbohydrate, quinine, anthraquinones, coumarins, phlobatanins, alkaloids, flavonoids, glycosides, terpenoids, diterpenes, phenols were found present while GC-MS analysis showed Eugenol, Cyclohexane, bicyclo[7.2.0]undec-4-ene, 4,11,11-trimethyl-8-methylene, Oxatricyclo[ 8.2.0.0(4,6)]dodecane,,12-trimethyl-9-methylene, Tetracontane, Phytol were present in majority. Conclusion: The study concluded that O. sanctum leaf extracts contain many biological active compounds which could be exploited for a development of plant based drug.

}, keywords = {Flavonoids, GC-MS Analysis, Herbal drugs, Phenolics, Phytochemical}, doi = {10.5530/pres.13.4.16}, author = {Atul Srivastava and Subhashini and Anand Kumar Keshari and Ragini Srivastava} } @article {702, title = {GC-MS Analysis of Bio-active Compounds in Methanolic Extract of Lactuca runcinata DC}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {December,2013}, pages = {58-61}, type = {Original Article}, chapter = {58}, abstract = {

Background: The presence of phytochemical constitutes has been reported from species of the Compositae (Asteraceae). Hitherto no reports exist on the phytochemical components and biological activity of Lactuca runcinata DC. Objective: The present study was designed to determine the bioactive compounds in the whole plant methanol extract of Lactuca runcinata. Materials and Methods: Phytochemical screening of the entire herb of Lactuca runcinata DC revealed the presence of some bio-active components. Gas chromatography-mass spectrometry (GC-MS) analysis of the whole plant methanol extract of Lactuca runcinata was performed on a GC-MS equipment (Thermo Scientific Co.) Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II. Results: The phytochemical tests showed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, phlobatannin, reducing sugars, saponins, steroids, tannins, terpenoids, volatile oils, carbohydrates, and protein/amino acids in methanolic extract of L. runcinata. The GC-MS analysis has shown the presence of different phytochemical compounds in the methanolic extract of Lactuca runcinata. A total of 21 compounds were identified representing 84.49\% of total methanolic extract composition. Conclusion: From the results, it is evident that Lactuca runcinata contains various phytocomponents and is recommended as a plant of phytopharmaceutical importance.

}, keywords = {GC-MS Analysis, Lactuca runcinata, Phytochemical screening, Whole plant methanol extract}, doi = {10.4103/0974-8490.122919}, author = {Lakshmi Kanta Kanthal and Akalanka Dey and K Satyavathi and P Bhojaraju} } @article {672, title = {Phytochemical Screening Studies on Melia Orientalis by GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {May 2013}, pages = {216-218}, type = {Original Article }, chapter = {216}, abstract = {

Background:Melia orientalis\ (MO) is an important Ayurvedic medicinal plants. The plant part such as leaves and roots are traditionally used for the treatment of diabetes, edema, traumatic swelling, skin diseases, oligospermia and bleeding disorders.\ Objective:\ To investigate the phytochemical identification of ethanol leaf extract of MO.\ Materials and Methods:\ The fresh leaves of MO (1000g) were collected and shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanol leaf extract obtained was dried and used for phytochemical identification by GC-MS analysis.\ Results:\ The phytochemical screening studies have been carried out and identified ten chemical constituents present in the leaf extract of MO.\ Conclusion:\ Thus, our results show that MO possess important phytocomponents such as phytol, squalene and stigmasterol.

}, keywords = {GC-MS Analysis, Medicinal l Pants, Melia Orientalis, Phytocomponents}, doi = {10.4103/0974-8490.112433}, author = {Srinivasan Marimuthu and Balakrishnan Padmaja and Sudarsan Nair} } @article {676, title = {GC-MS Analysis of Phytocomponents in the Ethanol Extract of Polygonum Chinense L.}, journal = {Pharmacognosy Research}, volume = {4}, year = {2012}, month = {december,2011}, pages = {11-14}, type = {Original Article}, chapter = {11}, abstract = {

Background: The presence of diverse secondary metabolites has been reported from species of the genus Polygonum. However, there has been not much information available on phytochemical components and biological activity in the whole plant ethanol extract of Polygonum chinense L. Objective: This study was designed to determine the phytocomponents in the whole plant ethanol extract of P. chinense. Materials and Methods: GC-MS analysis of the whole plant ethanol extract of P. chinense was performed using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i auto-sampler and a gas chromatograph interfaced to a mass spectrometer (GC-MS). Results: This investigation was carried out to determine the possible chemical components from P. chinense by GC-MS. This analysis revealed that the ethanol extract of P. chinense (whole plant) contained mainly a triterpene compound-squalene (47.01\%), and a plasticizer compound-1,2-benzenedicarboxylic acid, mono[2-ethylhexyl]ester (40.30\%). All identified compounds were, generally, reported as having antimicrobial activity. In addition, the squalene compound also having anti-cancer, anti-oxidant, anti-tumor, chemo-preventive, pesticidal and sun-screen properties, while the plasticizer compound -1,2-benzenedicarboxylic acid, mono[2-ethylhexyl] ester reported to have anti-oxidant and anti-inflammatory properties. No activity was reported in the alcoholic compound-4-hexene-1-ol, 5-methyl-2-(1-methylethanyl)-acetate-(R)-. Conclusions: From the results, it is evident that P. chinense contains various bioactive compounds and is recommended as a plant of phytopharmaceutical importance.

}, keywords = {GC-MS Analysis, Polygonum chinense, Rhytocomponents, Whole plant ethanol extract}, doi = {10.4103/0974-8490.91028}, author = {Bagavathi Perumal Ezhilan and Ramasamy Neelamegam} }