@article {1199, title = {Chemical Characterization of Two Botanicals from Genus Alternanthera - A. brasiliana (L.) Kuntze and A. paronychioides A. St.-Hil}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {42-50}, type = {Original Article}, chapter = {42}, abstract = {

Background: Traditional medicine has become part and parcel of the present era for the maintaining and preventing of ailments. Alternanthera brasiliana and Alternanthera paronychioides (Amaranthaceae) are widely used in traditional medicine. Ab is widely familiar as penicillin in Brazil. Ap is known to treat gout, hyperuricemia, rheumatic arthritis, nephritis etc. as folk medicine. The present study aims to compare standardization profiles for A. brasiliana and A. paronychioides. Materials and Methods: Sample Ab and Ap were collected and authenticated. Authenticated samples were subjected to powder microscopy, physico-chemical, phytochemical, HPTLC and HPLC fingerprint and PXRD analysis. Results: Powder microscopic investigations revealed the characteristic features for identification. Physico-chemical investigation revealed the slightly acidic nature of both plants. The phytochemical test showed the existence of phenol, terpenoids and steroids as major secondary metabolites in both species. Photo documentation, fingerprints and spectral comparison by HPTLC and fingerprints by HPLC validate the existence of similar compounds in both Ab and Ap. PXRD analysis revealed the variance of elements present in both species. Conclusion: Comparative physico-chemical, phytochemical and HPLC, HPTLC, and P-XRD instrumental analysis of A. brasiliana and A. paronychioides provides distinct features for identification.

}, keywords = {Amaranthaceae, chromatographic fingerprinting, Comparative standardization, HPLC, HPTLC, PXRD.}, doi = {10.5530/pres.16.1.6}, author = {Achintya Kumar Mandal and Rajesh Allu and Rajesh Chandran and Divya Kallingil Gopi and Sunil Kumar Koppala Narayana and Radha Prakasam and Shakila Ramachandran} } @article {666, title = {Corrected and Republished: Comparative Botanical and Phytochemical Studies of Ambiguous Medicinal Plant Species of Wedelia and Eclipta (Fam. Asteraceae) Used in ASU Systems of Medicine with Special Reference to in-silico Screening of Hepatoprotective Pote}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {April 2021}, pages = {54-67}, type = {Original Article}, chapter = {54}, abstract = {

Post the publication, as there were multiple corrections that were missed earlier, the article is being corrected and republished.

Comparative Botanical and Phytochemical Studies of Ambiguous Medicinal Plant Species of\ Wedelia\ and Eclipta (Fam. Asteraceae) Used in ASU Systems of Medicine with Special Reference to\ in-silico\ Screening of Hepatoprotective Potential of Marker Wedelolactone with Acetaminophen Targets.\ 

Context:\ In traditional medicine,\ Kesaraja\ (Ayurveda) or\ Manjal karisali\ (Siddha) is effective for jaundice.\ Aim:\ Three species of Asteraceae need to be studied for their therapeutic superiority of their intended claim. They are\ Wedelia chinensis\ (Osbeck) Merr. Philipp J.,\ Wedelia trilobata\ (L.) Hitchc. and\ Eclipta prostrata\ (L.) L. (Asteraceae). The present study aimed to screen and characterize the potential species for therapeutic purpose.\ Materials and Methods:\ The whole plants,\ W. chinensis\ (Osbeck) Merr.\ W. trilobata\ (L.) Hitchc. and\ E. prostrata\ (L.) (Asteraceae) were collected and botanically identified. Preliminary phytochemical analysis and high-performance thin-layer chromatography finger printing with marker wedelolactone were done for the ethanolic extracts of these plants. Botanical and pharmacognostical diagnostic characters of the plants based on macro-morphological, micro-morphological and powder microscopical characterization were worked out. Comparative\ in-vitro\ antioxidant potential of ethanolic extracts of these plant species was carried out. Using ADMET SAR software, the pharmacokinetics of wedelolactone were predicted. Using Autodock 4.2 software, the binding energy of wedelolactone on targets of acetaminophen-induced hepatotoxicity namely PPAR-α, AMPK, JNK-1, EGFR, Nrf2, ALT, ALP, GGT, CAR, Frizzled receptor, FXR, ERK1, LXR, mitochondrial glutamate dehydrogenase, p53, mTOR C1, CYP1A2, CYP2E1, 5-lipoxygenase, thrombin, UCP1, GSK1, RXR and PXR was predicted.\ Results:\ All the three plant species were pharmacognostically and chemically different.\ W. chinensis\ was found to possess more antioxidant potential than the other two plants. The marker compound wedelolactone was not detected in\ W. trilobata.\ Wedelolactone passed the Lipinski{\textquoteright}s rule of five, and the docking analysis of wedelolactone confirmed high binding affinity toward PPAR-α, AMPK, Nrf2, CYP2E1, EGFR, JNK1, UCP-2, thrombin, 5-lipoxygenase, mTORC1, RXR, FXR, LXR, Frizzled receptor, GDH and Erk-1.\ Conclusion:\ Based on the above observations, we conclude that the presence of marker compound wedelolactone might have attributed the potency of\ W. chinensis\ and\ E. prostrata\ in counteracting acetaminophen toxicity when compared with\ W. trilobata.

}, keywords = {Binding energy, botanical standardization, chromatographic fingerprinting, Docking, Hepatoprotective, in silico prediction, Lipinski{\textquoteright}s rule, Siddha, Therapeutic superiority, Wedelolactone}, doi = {10.4103/pr.pr_11_21}, author = {C. Arunachalam and R. Arunadevi and S. Murugamma and N. Monika and R. Susila and K. N. Sunil Kumar} }