@article {718, title = {Effect on eNOS/NO Pathway in MIRI Rats with Preconditioning of GFPC from Dang Gui Si Ni Decoction}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {March,2014}, pages = {133-137}, type = {Original Article}, chapter = {133}, abstract = {

Objective: In order to discover whether the eNOS/NO (endothelial nitric oxide synthase/nitric oxide) pathway is involved in the protective mechanisms of ischemic myocardium of DGSND (Dang Gui Si Ni Decoction) in MIRI (myocardial ischemia-reperfusion injury) SD rats. Materials and Methods: We made I/R (ischemia-reperfusion) model by ligating the left anterior-descending branch of the coronary artery (LAD) for 30 min and releasing the ligature for 120 min. eNOS (nitric oxide synthase) mRNA (message ribonucleic acid) and iNOS (inducible nitric oxide synthase) mRNA were measured by the methods of real-time RT-PCR (Real time Polychainase Chain Reaction), peNOS (phosphorylated eNOS) and iNOS protein were measured by the means of western blot. Results: In PPC group, real-time RT-PCR and western-blot analysis showed that eNOS mRNA and peNOS protein increased markedly (P \< 0.05); iNOS mRNA and protein decreased significantly (P \< 0.05). Conclusion: These results indicate that ischemic preconditioning (IPC) of GFPC from DGSND plays a protective role in I/R heart through regulating the eNOS/NO signal pathway, which could increase the eNOS gene expression and decrease the expression of iNOS mRNA.

}, keywords = {Cinnamic acid, eNOS, Ferulic acid, Glycyrrhizic acid, MIRI, NO, Paeoniflorin}, doi = {10.4103/0974-8490.129032}, author = {Guo-qiang Qian and Xia Peng and Chuan Cai and Guo-ping Zhao} } @article {631, title = {Modulation of Genotoxicity of Oxidative Mutagens by Glycyrrhizic Acid from Glycyrrhiza glabra L.}, journal = {Pharmacognosy Research}, volume = {4}, year = {2012}, month = {October ,2012}, pages = {189-195}, type = {Original Article}, chapter = {189}, abstract = {

Background: The chemopreventive effects of certain phytoconstituents can be exploited for their use as functional foods, dietary supplements and even as drugs. The natural compounds, acting as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological processes. Objectives: Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens. Materials and Methods: The methanol extract of Glycyrrhiza glabra rhizomes was subjected to column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay. Results: GLG fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative mutagens viz., H 2 O 2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester strain, it inhibited induction factor induced by H 2 O 2 and 4NQO by 75.54\% and 71.69\% at the concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced by H 2 O 2 and 4NQO by 70.21\% and 69.04\%, respectively, at the same concentration in human blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and was able to scavenge 85.95\% radicals at a concentration of 120 μM. Conclusion: Glycyrrhizic acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals.

}, keywords = {4NQO, Chemoprevention, Glycyrrhiza glabra L., Glycyrrhizic acid, H 2 O 2, Oxidative mutagens}, doi = {10.4103/0974-8490.102260}, author = {Prabhjit Kaur and Neha Sharma and Bikram Singh and Subodh Kumar and Satwinderjeet Kaur} }