@article {1217, title = {A Two-Year Comparative Study of the Anti-dermatophytic Activity of Thuja orientalis Essential Oil Stored at Various Temperatures}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {191-196}, type = {Original Article}, chapter = {191}, abstract = {

Background: Thuja orientalis generally recognized as Chinese arborvitae, placed under the family Cupressaceae is a commonly available medicinal plant in India. Objectives: In present research work Thuja leaves essential oil stored in three temperatures for two-years storage periods was evaluated against fungi causing ring worm infection in man. Materials and Methods: Thuja oil was hydro-distilled and stored for 24 months in airtight amber glass bottles at three distinct temperatures. MIC was determined through semi solid agar diffusion method containing BHIA medium. Anti-dermtophytic activity of stored oil was examined in triplets after every fourth month of storage up to 2 years. Results: Oil stored in refrigerator was found to be more stable up to two year of storage time. No activity changes were observed in this condition against all test fungi. While variable temperature condition of room temperature was found less suitable for the storage of oil. MIC against Candida albicans was increased 40\% after 2 years (\>3.5 μL/mL) and 66\% against Microsporum fulvum. Oil stored at 30{\textordmasculine}C temperature condition was found more stable upto IV testing. Slightly changes were observed during Vth testing against C. albicans, M. fulvum and M. canis. Conclusion: Present study concluded that essential oil of Thuja put away in chilliness condition can reserved their quality and properties of antifungal behaviour for long time. Accordingly, putting away of volatile oil ointment items in coolness (beneath 8{\textordmasculine}C) condition is best guidelines for long term use of therapeutic items.

}, keywords = {MIC., Microsporum, Thuja, Trichophyton, Volatile oil}, doi = {10.5530/pres.16.1.24}, author = {Neetu Jain and Seema Bhadauria} } @article {566, title = {Antidermatophytic Activity of Mikania micrantha Kunth: An Invasive Weed}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {s20-s25}, type = {Original Article}, chapter = {s20}, abstract = {

Context: The incidence of dermatophytosis has risen dramatically in recent years. Limited availability of side-effect free drugs has led to a search for new antidermatophytic agents. Objective: The objective was to investigate antidermatophytic activity and in vitro anti-inflammatory activity (protease inhibition assay) of whole plant (aerial parts only) of Mikania micrantha. Materials and Methods: The dried and powdered aerial parts of M. micrantha were extracted separately with petroleum ether, ethyl acetate and methanol. Antidermatophytic activity was determined by agar tube dilution method against Epidermophyton floccosum var. nigricans, Microsporum canis, Microsporum gypseum and Trichophyton rubrum. The activities of various parts of the plant - flowers, leaves and stem were separately analyzed using their ethyl acetate extract. Fungicidal efficacy and trypsin inhibiting activity of the whole plant, flowers and leaves were also analyzed using the ethyl acetate extracts. Statistical Analysis Used: For trypsin inhibition assay results are expressed as mean {\textpm} standard division. For antidermatophytic assay, the significance of the difference between control and test was analyzed statistically using Fisher{\textquoteright}s exact test. Results: Ethyl acetate extract of M. micrantha exhibited excellent antidermatophytic activity, followed by petroleum ether and methanolic extracts. Ethyl acetate extracts of whole plant, flowers, leaves and stem completely inhibited the growth of dermatophytes at the tested concentration of 2 mg/mL. Furthermore, ethyl acetate extracts of whole plant, leaves and flowers were fungicidal, and the percentages of trypsin inhibition exhibited were 33.73 {\textpm} 0.306, 39.0 {\textpm} 0.505 and 35.53 {\textpm} 0.503, respectively. Conclusions: Since M. micrantha possesses antidermatophytic as well as anti-inflammatory activities, the plant is an excellent candidate for the development of new medicaments against dermatophytoses in traditional as well as modern medicine.

}, keywords = {Anti-inflammatory action, Epidermophyton, Microsporum, Trichophyton, Trypsin inhibiting assay}, doi = {10.4103/0974-8490.157994}, author = {Madhavankutty Jyothilakshmi and Mathew Jyothis and Mukalel Sankunni Latha} }