@article {1453, title = {Fabrication of Snail Mucin-Silk Fibroin Hydrogel Scaffold for Guided Tissue Regeneration: A Zebrafish Fin Model Study}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {927-933}, type = {Original Article}, chapter = {927}, abstract = {

Background: Scaffolds play a pivotal role in guiding tissue regeneration by providing a suitable environment for cell growth, migration, and differentiation. Among various materials, biopolymers such as silk fibroin and snail mucin have garnered attention due to their biocompatibility, natural origin, and potential to promote cellular activities. Objectives: In this study, we explore the fabrication of a novel Snail Mucin-Silk Fibroin hydrogel scaffold designed to enhance tissue regeneration. Materials and Methods: The hydrogel scaffold has been prepared by mixing 28\% w/v gelatin, 21\% w/v PVA in distilled water and 10\% HPMC, and heated to 40{\textordmasculine}C followed by addition of a snail mucin and silk fibroin at a lower temperature and freeze dried at 4{\textordmasculine}C for 12 hr. Results: The characterization tests showed the scaffold{\textquoteright}s favourable biological properties and morphology, such as biocompatibility, hemocompatibility, ability to retain moisture, and promotion of cellular activities essential for tissue repair. Histopathological analysis showed that the snail mucin-silk fibroin hydrogel scaffold significantly enhanced fin regeneration compared to the control groups. Conclusion: Thus, the snail mucin hydrogel used in this study showed better soft tissue regeneration potential and hence be used for various applications in regenerative medicine.

}, keywords = {Hydrogel, Regeneration, Silk Fibroin, Snail Mucin, Zebra Fish}, doi = {10.5530/pres.20252176}, author = {Sofia Sebastian and Bargavi Purushothaman and Pratibha Ramani} } @article {564, title = {The evaluation of the activity of medicinal remedies of plant and animal origin on the regeneration of the earthworms{\textquoteright} tail segments}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {October 2015}, pages = {393}, type = {Original Article}, chapter = {393}, abstract = {

Now, in the global community there is enough hard recommendation to replace the vertebrate test animals into simpler organisms at the development, testing, and evaluation of the quality pharmaceuticals. The feature of planarian to regenerate in new individual planarian from a piece, which is only 1/7 of the original animal, allowed to create the alternative methods of testing of drugs, dietary supplements, water quality, influence of electromagnetic fields, and other radiations. The tests on planarian can replace the ones that are held today on mammals. However, the lacks of the bioassays based on the planarian regeneration are the need for complex and expensive video equipment for recording the regrowth of worms{\textquoteright} body, the difficulties of culturing of flatworms and fairly long period of response. These difficulties can be avoided by using another group of the worms of type Annelida. The new individual can be fully recovered only from the front half of the body in many species of earthworms. Thus, the influence of the pharmaceuticals from earthworms, mummy, and Orthilia secunda on the ability of earthworms to regenerate lost tail segments has been investigated. The relations of the activity of preparations tested with doses and the time of the storage have been revealed. The principal possibility of applicability of the test reaction studied as a way to evaluate the effects and quality of remedies based on medicinal plants and earthworms has been shown.

}, keywords = {Earthworms, Medicinal remedies, Mummy, Orthilia secunda, Regeneration, Test-reaction, Vermipreparation.}, doi = {10.4103/0974-8490.159581}, author = {Viktor Alexandrovich Bybin and Daevard Iosifovich Stom} }