@article {1110, title = {Monitoring of Cannabis Cultivar Technological Maturity by Trichome Morphology Analysis and HPLC Phytocannabinoid Content}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {December 2022}, pages = {94-100}, type = {Original Article}, chapter = {94}, abstract = {

Objectives: This study aims at determining technological maturity (ten, seven, two days prior and at harvest day) through analyzing morphological changes of trichomes and phytocannabinoid content in samples from cultivated medical cannabis at three plot spots (southeast - SE, central - CN and northwest - NW) in greenhouse (with 3600 m2). Materials and Methods: Soil-grown medical cannabis T-492 plant flowers were sampled within a ten days{\textquoteright} timeline from three plot spots of 3600 m2 greenhouse. Morphology analysis was performed using Zeiss Stemi 508 stereomicroscope and phytocannabinoids content assessment was performed using German pharmacopoeial method for assay of cannabinoids. Results: HPLC analysis revealed that total THC (\%) in the samples was declining from SE (13.14\%), all the way through CN (12.38\%) to NW (9.14\%) spot. Regarding timeline, total THC (\%) was the highest in the southeast location, starting with the highest levels on the tenth day before harvest (SE-10- 13.14\%) and then decreasing on the seventh day prior to harvest (SE-7 -11.78\%), on the second day prior harvest (SE-2-11.56\%) and at the harvest day (SEh- 10.00\%). The presence of translucent-milky, yellow-orange and dark brown trichome heads was observed. An increment of the number of yellow-orange and dark brown colored capitate-stalked trichome heads was connected with cannabinoid content declining. Conclusion: This research gives directions for future conceptualization of correlational studies of cannabinoid content and changes in trichomes and purple coloration (probably from anthocyanins) in different Cannabis cultivars.

}, keywords = {Cannabinoid potency, Cultivation, Flowers, Harvest, Hemp, Liquid chromatography, Trichome morphology.}, doi = {10.5530/097484900306}, author = {Veronika Stoilkovska Gjorgievska and Ivana Cvetkovikj Karanfilova and Ana Trajkovska and Marija Karapandzova and Biljana Bauer Petrovska and Svetlana Kulevanova and Gjose Stefkov} } @article {518, title = {Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {281-286}, type = {Original Article}, chapter = {281}, abstract = {

Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.

}, keywords = {Benincasa hispida, Carissa congesta, Liquid chromatography, Mass spectroscopy, Polyalthia longifolia, Quercetin, Rutin}, doi = {10.4103/0974-8490.186578}, author = {Gaurav M. Doshi and Vivek V. Nalawade and Aaditi S. Mukadam and Pratip K. Chaskar and Sandeep P. Zine and Rakesh R. Somani and Hemant D. Une} }