@article {85, title = {Evaluation of Phytochemical Content of White Tea Clone 100 and Changes the Expression of Tumor Suppressor Genes on Colorectal Cancer Cell Line HCT116}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {224-229}, type = {Original Article}, chapter = {224}, abstract = {

Aim/Background: Colorectal carcinoma cancer is one of the main types of cancer with high death rate of patients, according to their non-healthy lifestyle. In this research, we evaluated inhibitory of white tea clone 100 extract on colorectal cancer cell line HCT-116, and its effectiveness in expression level of three tumor suppressor genes. Materials and Methods: Total polyphenolic content in all samples were measured using the Folin{\textendash}Ciocalteu method, and free radical adsorption investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the white tea extract inhibitory effect on HCT-116 cells. Results: Among different extraction methods, the white tea aqueous extracts produced the highest amount of polyphenols and DPPH radical scavenging activity (36.67 {\textpm} 0.54 mg gallic acid equivalent/g dry weight and 71.74\% {\textpm} 0.42\%, respectively). Cell survival analysis in the MTT assay indicated that aqueous white tea extract could reduce the viability of HCT-116 cells in 8, 16, and 24 h considerably, related to the concentration-dependent manner. The real-time polymerase chain reaction results indicated the significantly increased expression level (P \< 0.05) of tumor suppressor genes DCC, TGFBR2, and P53 in the concentration of 1000 μg/ml at 24 h. Conclusion: White tea aqueous extract because of having hydroxyl group in its structure at optimal concentration in all three times of experimental, could lead to a positive changes effect on gene expression in the tumor suppressor family.

}, keywords = {3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, HCT-116 cell line, Radical scavenging activity, Tumor suppressor genes, White tea}, doi = {10.4103/pr.pr_39_19}, author = {Fereydoon Bondarian and Asa Ebrahimi and Frouzandeh Mahjoubi and Eslam Majidi Hervan and Reza Azadi Gonbad} } @article {395, title = {Chemical Constituents and Bioactivities of Glinus Oppositifolius}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {138-147}, type = {Original Article }, chapter = {138}, abstract = {

Objectives: To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius. Methods: The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail-flick assay, and antimicrobial potential using the disc diffusion method. Results: The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced \% blood glucose reduction of 70.76\% {\textpm}17.4\% within 0.5 h after introduction. The DCM leaf extract showed a lower \% blood glucose reduction of 18.52\% {\textpm}13.5\% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens. Conclusion: The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.

}, keywords = {3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Analgesic, Antimicrobial, Cytotoxic, Glinus oppositifolius Linn, Hypoglycemic, Molluginaceae, Oppositifolone}, doi = {10.4103/0974-8490.150520}, author = {Consolacion Y. Ragasa and Esperanza C. Cabrera and Oscar B. Torres and Adiel Inah Buluran and Dinah L. Espineli and Dennis D. Raga and Chien-Chang Shen} }