@article {1214, title = {In silico Based Virtual Screening of Non-polar Phytochemicals obtained From Petroleum Ether Extract of Asparagus racemosus by GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {161-171}, type = {Original Article}, chapter = {161}, abstract = {

Background: Evidence shows that people have used medicinal plants and plant components to treat various conditions since ancient times. Asparagus racemosus have a wide range of therapeutic potential Antioxidant, anti-cancer, anti-fungal, anti-bacterial, and anti-inflammatory. Aim and Objectives: The current study sought to identify potential bioactive molecules present in the petroleum ether extract of Asparagus racemosus. Materials and Methods: The presence of different molecules was identified by Gas Chromatography. Mass spectroscopy and FTIR analysis confirmed the molecular structure. The identified molecule was analyzed for drug likeliness, bioactivity, and target prediction analysis by in silico approaches using Swiss ADME, Swiss target prediction, Oasis datawarior, and mole inspiration software. Results: The petroleum ether extract is the highest source of alkaloids and steroids. The molecule (5.beta.) Pregnane-3,20, beta.-diol, 14.alpha.,1, 8. alpha.-[4-methyl-3-oxo-(1-oxa-4-azabutane-1,4-diyl)]- diacetate was found in the highest concentration (37.33\%). The identified molecule is steroidal. The in silico study exhibited good drug likeliness and therapeutic potential for the reported molecule. The molecule can be considered a potential therapeutic agent for the treatment of cancer and analgesic anti-inflammatory agents. Conclusion: The molecule (5.beta.) Pregnane-3, 20. beta.-diol, 14.alpha.,1, 8. alpha.- [4-methyl-3-oxo-(1-oxa-4-azabutane-1,4-diyl)]-,diacetate can be considered as poteintial therapeutic agent.

}, keywords = {Asparagus racemosus, Drug likeliness, Gas chromatography, In silico study, Mass spectroscopy, Petroleum ether, Target prediction.}, doi = {10.5530/pres.16.1.21}, author = {Shaik Harun Rasheed and Ravi Kumar Vakkalagadda and Sayyad Mustak and Sandip Sen} } @article {1213, title = {Isolation, Purification and Characterization of L-Carvone from Mentha longifolia Using Fractional Distillation and Quantified by Gas Chromatography}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {156-160}, type = {Original Article}, chapter = {156}, abstract = {

Menta longifolia is an aromatic herb consists of dried aerial parts of Mentha longifolia belongs to family Lamiaceae. Overground parts of the plant yield 2-4\% of an essential oil, of which the chief component appears to be the monoterpene ketone, carvone. Mentha longifolia thus approaches spearmint (Mentha spicata) rather than peppermint (M. piperita) in oil composition. Other major constituents of the essential oil, according to various studies, include piperitenone and its oxide, piperitone and its oxide and pulegone. The present study involves isolation of l-Carvone from Mentha longifolia using fractional distillation as a tool for separation which was quantified using Gas chromatography. Characterization of purified L-Carvone was done using NMR spectroscopy (1HNMR and 13C-NMR). Fractions collected using fractional distillation was evaluated for anti-microbial activity for further studies.

}, keywords = {Gas chromatography, L-Carvone, Mentha longifolia, NMR.}, doi = {10.5530/pres.16.1.20}, author = {Hayat M. Mukhtar and Nishant Kumar and Amandeep Singh and Abhay Sharma and Priyanka and Prince Ahad Mir} } @article {471, title = {Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June,2015}, pages = {282-293}, type = {Original Article}, chapter = {282}, abstract = {

Background: Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries. Objectives: Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography. Materials and Methods: Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents. Results: The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5-hydroxy, 2,4-butylphenyl; n-Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2-ethylhexylhepatdecyl ester; n-Tridecane; 6-methyltridecane; (9E, 12E)-9,12-Octadecadienyl chloride, Hexadecanoic acid, 3-(trimethylsilyl)-oxy] propyl ester; 9,12-Octadecadenoic acid, 2 hydroxy-1-(hyroxymethylethyl) ester; 9,12-Octadecadienoic acid, 2,3 dihydroxypropyl ester; n-Propyl-9,12-Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a-Methanopicene, perhydro-12,4a, 61a, 9,9,12a-hepatmethyl-10-hydoxy and 9-Octadecene; 2-Isoprpenyl-5-methyl-6-hepten-1-ol; n-Hexadecanoic acid, 2-hyroxy-1-(hydroxymethyl) ethyl ether; Butyl-9,12-Octadecadieonate; Friedoolean-8-en-3-one; friedours-7-en-3-one; 13,27-Cyclosuran-3-one; Stigmaste-7,25-dien-3-ol (3β, 5α); Stigmasta-7,16-dien-3-ol; chrondrillasterol in BH seeds and CC roots extracts respectively. Conclusion: Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.

}, keywords = {25-dien-3-ol., Apocynaceae, Benincasa hispida, Carissa congesta, Chrondrillasterol, Cururbitaceae, Gas chromatography, Lupeol, Mass spectroscopy, Stigmaste-7}, doi = {10.4103/0974-8490.157179}, author = {Gaurav M. Doshi and Vivek V. Nalawade1 and Aaditi S. Mukadam and Pratip K. Chaskar and Sandeep P. Zine and Rakesh R. Somani and Hemant D. Une} }