@article {1336, title = {Ethanolic Extract of aerial parts of Achyranthes aspera Attenuates Hepatotoxicity and Nephrotoxicity Induced by Cisplatin in Rat}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {60-69}, type = {Original Article}, chapter = {60}, abstract = {

Background: The chemotherapeutic agents used to treat cancer are known to possess potent adverse effects like nephrotoxicity and hepatotoxicity among others. Objectives: The aim of this current study happened to be the isolation of phytoconstituent from Achyranthes aspera and assess the protective effect of the compound against cisplatin-induced hepato- and nephrotoxicity in rats. Materials and Methods: Toluene: Ethyl Acetate (9:1) was used as the solvent system to isolate the phenolic compound from ethanolic extract of the leaves and stem of A. aspera. The isolated compounds were studied using mass spectroscopy, 1H NMR, FT-IR and UV. The effect of the isolated phenolic was assessed by study of various factors like body weight, organ weight, serum biomarkers and histopathological studies. Results: The treatment of cisplatin led to a significant increase the weight of the organs, hepatic and kidney biomarkers in serum suggesting toxicity whereas the the isolated component reversed the elevated levels (p\<0.05 compared to positive control). Conclusion: It could be concluded from the results that pretreatment with isolated component (coumarin) was able to attenuate the hepato-and nephron-toxicity induced by anticancer drug cisplatin.

}, keywords = {Achyranthes aspera, Cisplatin, Hepatotoxicity, Isolation, Nephrotoxicity}, doi = {10.5530/pres.20251905}, author = {Arijit Chaudhuri and Udichi Kataria and Anurag Dubey} } @article {1239, title = {A Comprehensive Study on Achyranthes aspera and Aesculus indica Hepatoprotective and Nephroprotective Activities of against Cisplatin Induced Rodent Model}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {April 2024}, pages = {315-323}, type = {Original Article}, chapter = {315}, abstract = {

Aim: In our present research, we aimed to evaluate the protective effects of HF1, HF2, and HF3 against liver and kidney damage induced by Cisplatin in Sprague Dawley rats. The ethanol fraction of the plant underwent initial phytochemical analysis followed by subsequent in vivo screenings. The findings from the phytochemical investigation indicated that HF2 demonstrated a more potent hepatoprotective and renal protective activity. Background: Anti-cancer medications have the potential to inflict harm on the liver and kidneys, underscoring the crucial need for hepatoprotection in safeguarding liver function. Among the elderly population, liver and kidney impairment account for 10\% of chronic hepatitis and renal failure cases. The decline induced by anti-cancer drugs has not been thoroughly assessed, partly due to its underestimation in clinical practice and challenges in making a precise diagnosis. While chemotherapeutic agents can induce liver toxicity through various mechanisms and lead to different types of liver and kidney injuries, they share the common trait of being both hepatotoxic and nephrotoxic. Materials and Methods: The dried peels of Aesculus indica fruits and powdered leaves of Achyranthes aspera were subjected to Soxhlet extraction using 95\% ethanol. Rat will randomly into five groups (six rats per each group). cisplatin group will give a single injection of cisplatin (20 mg per Kg, i.p) on the first 1st, 7th, 14th, 21th and 28th days. Hepatic function was assessed by measuring blood bilirubin, Glutamate Oxaloacetate Transferase (SGOT), and Glutamate Pyruvate Transaminase (SGPT). Blood tests were used to check the function of the liver and kidneys. Results: These results collectively suggest that HF1, HF2, and HF3 serve as a potential reservoir of novel secondary metabolites. Moreover, this study has laid the groundwork for future research in pharmacological studies, particularly in the development of potent biomedicine for the treatment of various chronic diseases. Conclusion: The current study has also proved the way for future pharmacological investigations, particularly with regard to all medications derived from the creation of powerful medicines for the management of various chronic conditions.

}, keywords = {Cisplatin, Hepatoprotective, Herbal formulation., nephroprotective}, doi = {10.5530/pres.16.2.40}, author = {Arijit Chaudhuri and Udichi Kataria and Shubham Anand} } @article {1283, title = {Evaluation of Balya Activity of Atibala (Abutilon indicum (Linn.) Sw) in Cisplatin-Induced Muscle Atrophy Model}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {733-740}, type = {Original Article}, chapter = {733}, abstract = {

Background: Cisplatin, an antineoplastic drug, induces muscle atrophy in patients. Given this, investigating potential interventions such as nutritional supplements or medications to regulate catabolic processes, cellular damage and inflammatory responses emerges as a promising strategy for restoring muscle mass and strength. Drawing from the rich tradition of Ayurveda, Atibala (Abutilon indicum (Linn.) Sw) is noted in the Balya Dashemani of Caraka Samhita for its reputed role in promoting muscle bulk and strength. In light of this, we undertook the present study to evaluate the potential of Atibala root decoction in enhancing health and counteracting muscle weakness induced by cisplatin in Wistar rats. Objectives: The primary aim was to evaluate the health-promoting (balya) activity of Atibala (Abutilon indicum (Linn.) Sw) in an animal model. Additionally, we sought to assess its effectiveness in countering cisplatin-induced muscle weakness in the same animal model. Materials and Methods: 32 male Wistar rats, each weighing 150-200 g, were divided into five groups. Muscle weakness was induced by intraperitoneal injections of cisplatin on days 1, 8 and 15. Atibala Kashaya was administered daily. Muscle mass, strength, body weight, thigh circumference, running time on the rotarod, distance traveled in the actimeter and serum cortisol levels were assessed. Gastrocnemius muscle histopathology was also examined upon sacrifice at the end of the study. Results: Histopathological analysis revealed improved muscle growth in the Atibala Kashaya-treated groups. While no significant differences were observed in serum cortisol, rotarod and actimeter parameters between the groups, a mean difference was noted on Day 15 and Day 45. Significant improvements in weight and thigh circumference were found when analyzed within all groups.

}, keywords = {Atibala, Balya, Cisplatin, Muscle Atrophy, Weakness}, doi = {10.5530/pres.16.4.85}, author = {Vishala Hiremath and Vedantam Giridhar and Santosh F Patil and Ranjit Kangle} } @article {448, title = {Protective effect of Withania coagulans fruit extract on cisplatin-induced nephrotoxicity in rats}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {November 2017}, pages = {354-361}, type = {Original Article}, chapter = {354}, abstract = {

Background: Fruits of Withania coagulans (Solanaceae) reported to possess several bioactive compounds as curative agents for various clinical conditions. Cisplatin is a chemotherapeutic drug to treat sarcomas, carcinomas, lymphomas, cervical cancer, germ cell tumors, etc. The major factor that limits its clinical use is its dose-dependent nephrotoxicity. Aim: To explore the nephroprotective effect of W. coagulans extract and its modulatory effects against cisplatin-induced nephrotoxicity and genotoxicity. Materials and Methods: W. coagulans fruit extract was quantitatively standardized with withaferin A using high-performance thin-layer chromatography. The subacute toxicity study was performed according to OECD guidelines in experimental rats. Nephrotoxicity in rats was induced by a single dose of cisplatin (6 mg/kg, intraperitoneal). Nephroprotective role of W. coagulans fruit extract at different doses had been evaluated. It includes quantification of serum kidney toxicity markers, renal tissue oxidative stress biomarkers and pro-inflammatory cytokines level, DNA fragmentation assay, and histopathological examination of renal tissue. Results: Withaferin A was found 3.56 mg/g of W. coagulans fruit extract. It significantly prevented the rise in serum urea and creatinine level and also preserve rat kidneys from oxidative stress and free radical induced DNA damage. Histopathological study showed extract treatment eliminates tubular swelling, cellular necrosis, and protein cast deposition in cisplatin treated kidney tissue. It averted the decline in glutathione content, activities of superoxide dismutase and catalase. These parameters were restored to near normal levels by extract in a dose of 400 mg/kg, per oral. Conclusion: It can be justified that W. coagulans possess dose dependent protective effect against cisplatin induced kidney damages, primarily through its free radical scavenging and anti inflammatory activity

}, keywords = {Cisplatin, DNA fragmentation assay, nephroprotective, withaferin A, Withania coagulans}, doi = {10.4103/pr.pr_1_17}, author = {Sonam Sharma and Apurva Joshi and Siva Hemalatha} } @article {483, title = {Ameliorative Effects of Chloroform Fraction of Cocos nucifera L. Husk Fiber Against Cisplatin-induced Toxicity in Rats}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {February 2016}, pages = {89-96}, type = {Original Article}, chapter = {89}, abstract = {

Background: Cisplatin (Cis) is used in the treatment of solid tumors and is known to elicit serious side effects. Objective: The present study investigated the protective effects of chloroform fraction of Cocos nucifera husk fiber (CFCN) against Cis-induced organs{\textquoteright} damage and chromosomal defect in rats. Quercetin (QUE), standard antioxidant, served as positive control. Materials and Methods: Thirty male Wistar rats were assigned into six groups and treated with corn oil (control), Cis alone, Cis + CFCN, CFCN alone, Cis + QUE, and QUE alone. QUE and CFCN were given at 50 and 200 mg/kg/day, respectively, by oral gavage for 7 days before the rats were exposed to a single dose of Cis (10 mg/kg, intraperitoneal) at the last 36 h of study. Results: Administration of Cis alone caused a significant (P \< 0.05) increase in the levels of serum creatinine and urea by 72\% and 70\%, respectively, when compared with the control. The activity of serum aspartate aminotransferase was significantly (P \< 0.05) increased while alanine aminotransferase and alkaline phosphatase were insignificantly (P \> 0.05) affected in Cis-treated rats. Furthermore, the activities of hepatic and renal catalase, superoxide dismutase, glutathione S-transferase, glutathione peroxidase, and levels of reduced glutathione were significantly (P \< 0.05) decreased in Cis-treated rats with concomitant elevation of malondialdehyde. Cis exposure increased the frequency of micro nucleated polychromatic erythrocytes (mPCE) by 92\%. Pretreatment with CFCN inhibited lipid peroxidation, enhanced the activities of some antioxidative enzymes and reduced the frequency of mPCE. Conclusions: Chloroform fraction of CFCN may protect against organs damage by Cis. Further studies are required to determine the component of the plant responsible for this activity.

}, keywords = {Antioxidant, Cisplatin, Clastogenicity, Cocos nucifera, Lipid peroxidation}, doi = {10.4103/0974-8490.172658}, author = {Oluwatosin Adekunle Adaramoye and Adesola Fausat Azeez and Olufunke Elizabeth Ola-Davies} }