@article {1133, title = {Essential Oils of Rosmarinus officinalis and Eucalyptus globulus Cultivated in the Mountainous Region of the State of Rio de Janeiro (Brazil): Chemical Profile and Antileishmanial Activity}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {297-306}, type = {Original Article }, chapter = {297}, abstract = {

The present study aimed to characterize the chemical profile of essential oils of Rosmarinus officinalis (EORo) and Eucalyptus globulus (EOEg) cultivated in the mountainous region of the state of Rio de Janeiro, Brazil, and to verify the leishmanicidal potential, as well as to determine the cytotoxicity in vitro in mammalian cells. EORo had α-Pinene (37.99\%), Cineol (21.48\%), D-Verbenone (5.78\%) and Limonene (3.28\%) as major constituents. The EOEg had as major components Cineol (75.52\%), Limonene (8.82\%) and o-Cymeno (7.81\%). The EOEg had a lower IC50 value of 14.03 {\textpm} 2.08 μg/mL, while EORo had an IC50 value of 31.12 {\textpm} 4.6 μg/mL, both during the 24-hr exposure period. In the 48-hr period, the IC50 value was almost twice as high with rosemary oil, while in eucalyptus oil there was no significant difference when exposed in this period. The selectivity of EOs was demonstrated in murine cells, showing almost 30 times less toxic to the cell than to the parasite. It can be concluded that this is a pioneering study on the potential of these essential oils against the leishmania parasite. Furthermore, the low toxicity effect on the host cell encourages future studies and in vivo applications.

}, keywords = {cytotoxicity, E. globulus, Essential oil, Leishmanicidal, R. officinalis}, doi = {10.5530/pres.15.2.032}, author = {Rog{\'e}ria Cristina Zauli and Tarc{\'\i}sio Martins Santos and Alberto Wisniewski Jr and Patr{\'\i}cia Xander and Aislan Cristina Rheder Fagundes Pascoal} } @article {413, title = {Anti-Leishmania Activity of Osthole}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {March 2016}, pages = {1-4}, type = {Original Article}, chapter = {1}, abstract = {

Background: Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to improve the current regimens. Objective: In this study in vitro and in vivo antileishmanial activities of osthole, a natural coumarin known to possess antibacterial and parasiticidal activities are evaluated. Materials and Methods: Leishmania major infected J774.A1 macrophages were treated with increasing concentrations of osthole. CL lesions of BALB/c mice were treated topically with 0.2\% osthole. Results: Osthole exhibited dose-dependent leishmanicidal activity against intracellular amastigotes with IC50 value of 14.95 μg/ml. Treatment of CL lesions in BALB/c mice with osthole significantly declined lesion progression compared to untreated mice (P \< 0.05), however did not result in recovery. Conclusion: Osthole demonstrated remarkable leishmanicidal activity in vitro. Higher concentrations of osthole may demonstrate the therapeutic property in vivo.

}, keywords = {Cutaneous leishmaniasis, Leishmania major, Leishmanicidal, Osthole}, doi = {10.4103/0974-8490.178650}, author = {Elaheh Kordzadeh Kermani and Seyed Ebrahim Sajjadi and Seyed Hossein Hejazi and Reza Arjmand and Sedigheh Saberi and Abbas Ali Eskandarian} }