@article {387, title = {Methanolic Extract of Curcuma caesia Roxb. Prevents the Toxicity Caused by Cyclophosphamide to Bone Marrow Cells, Liver and Kidney of Mice}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {December 2015}, pages = {43-49}, type = {Original Article}, chapter = {43}, abstract = {

Introduction: With an ever increasing cause of cancer, it has been recommended to treat with conventional drugs, however because of the side effects caused by the conventional drugs, the research on medicinal plants has been intensified due to their less adverse and toxic effects. Objectives: The primary objective of the present study was to evaluate the protective effect of the medicinal plant Curcuma caesia Roxb. against free radicals ABTS+ and O2 -. Also it was aimed to evaluate the protective effect of C.caesia Roxb. against the chemotherapeutic drug Cyclophosphamide and its side effects in liver and kidney. Methods: The rhizomes of the plant was extracted with methanol through soxhlet and its antioxidant activity was tested against ABTS+ and O2 - . For antigenotoxic studies, animals were divided into eight groups and micronucleus assay was employed and for biochemical analysis serum sample was collected from the blood and SGOT, SGPT analysis was performed. Also the biochemical analysis was performed from both the liver and kidney. Results: The methanolic extract of Curcuma caesia Roxb. was found to scavenge the free radicals ABTS+ and O2 - . the micronuclei formation was found to be increased in the positive control group as compared to the negative control group significantly (P\<0.002) however increase in the number of micronuclei was found to be decrease with the pretreatment of the extract at different concentrations significantly as compared to the negative control groups (P\<0.01, P\<0.005,P\<0.001). The increased level of serum SGPT and SGOT as well as peroxidation level in both liver and kidney due to treatment of cyclophosphamide was also found to be decreased with the pretreatment of the extract significantly as compared to the positive control groups. There was decreased in the level of endogenous antioxidant such as GSH and GR in the positive control group however decreased level of GSH and GR was found to be increased with the pretreatment of the methanolic extract of C. caesia Roxb. Conclusion: The present study suggested that the methanolic extract of C. caesia Roxb has not shown any genotoxicity and reduces the genotoxicity caused by cyclophosphamide. It was also to have the protective effects against the liver and kidney. So it could be provided as one of the herbal supplementation in chemoprevention of CP to ameliorate the side effects of it.

}, keywords = {2, 2{\textquoteright} azino bis (ethylbenzthiazolene-6-sulfonic acid) radical cation assay, Curcuma caesia Roxb., Cyclophosphamide, micronucleus assay, Oxidative stress, Superoxide dismutase assay}, doi = {10.4103/0974-8490.171106}, author = {Heisanam Pushparani Devi and Pranab Behari Mazumder} } @article {602, title = {Dietary Supplementation with two Lamiaceae Herbs-(oregano and sage) Modulates Innate Immunity Parameters in Lumbric us Terrestris}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {January 2013}, pages = {1-9}, type = {Original Article }, chapter = {1}, abstract = {

Introduction:Lamiaceae\ herbs have are well known for their immunomodulatory effects, however, the mechanism by which they effect innate immune system is not clearly understood.\ Objective:\ The effect of dietary supplementation with two\ Lamiaceae\ herbs (oregano and sage) modulation of on innate immunological parameters was investigated in\ Lumbricus terrestris.\ Materials and Methods:\ Animals were fed (ad libitum) on herbs supplemented diet [(0.1\% (w/v) and 0.5\% (w/v)] for 6 days. Changes in immune competent cell counts, viability, and relative neutrophil-like cell counts were determined in response to herb treatment. Changes in nitric oxide, phagocytic activity, and respiratory burst index were also determined in response to herb treatment relative to control. Additionally, effect of herb co-treatment cyclophosphamide (50 mg/kg-BW) induced immunosuppression was also evaluated.\ Results:\ Our results suggested abrogation of CP-induced immunosuppression in response to co-treatment with herbs. Significant increase in nitric oxide-mediated immune-competent cell counts, viability, and differentiation into neutrophil-like cells were observed in response to dietary supplementation with\ Lamiaceae\ herbs. Significantly higher phagocytic activity relative to control was also noted in response to dietary intake of oregano and sage. However, the respiratory burst index did not increase exponentially in response to herb treatments, suggesting a potential enhancement in pathogen recognition and antioxidant defenses.\ Conclusion:Lamiaceae\ herbs may have potential immune-modulatory properties important for human health and merits further investigation.

}, keywords = {Cyclophosphamide, Iimmunosuppression Innate Iimmunity, Respiratory Burst, Zingiberaceae Herbs}, doi = {10.4103/0974-8490.105636}, author = {DA Vattem and CE Lester and RC DeLeon and RC DeLeon and RC DeLeon} }