@article {993, title = {In-silico Molecular Docking Studies of some Isolated Phytochemicals from Biophytum veldkampii against Cyclooxygenase-II Enzyme and in vivo Anti-inflammatory Activity}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {October 2021}, pages = {192-198}, type = {Original Article}, chapter = {192}, abstract = {

Background: The current study estimate the anti-inflammatory activity of whole plant of Biophytum veldkampii on carageenan induced inflammation on rats compiled by molecular docking studies of phytocompounds from the plants with Cyclooxygenase II (PDB ID: 3LN1). Methods: In this research Biophytum veldkampii was subjected to extraction using methanol. In-vivo anti-inflammatory activity was assessed by using carageenan induced inflammation on rats and in-silico molecular docking studies was performed by using of Autodock 4.0. Results: The outcomes revealed that the methanolic extract has most prominenant anti-inflammatory activity at various doses. Among all the substances 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone revealed the most effective docking rating of -6.8, which is near to Diclofenac, i.e. - 6.9, ensuring that 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone has a strong binding fondness in between protein and ligand. Conclusion: From the results, a conclusion can be drawn that the anti-inflammatory activity of Biophytum veldkampii in both in vivo and in silico methods. This information sustains 2,6,3{\textquoteright},4{\textquoteright}-Tetrahydroxy-2-benzylcoumaranone to be a useful anti-inflammatory compound beneficial to future clinical studies.

}, keywords = {2, 3{\textquoteright}, 4{\textquoteright}-Tetrahydroxy-2 benzylcoumaranone, 6, Autodock4.0, Carageenan, Cyclooxygenase II, In-silico, Inflammation, Phytochemical}, doi = {10.5530/pres.13.4.11}, author = {Nagaraju Bandaru and D.S.N.B.K Prasanth and A.Rajasekhar Reddy and G.S.N Koteswara Rao and Kumar V.S Nemmani and Alla Narayana Rao} } @article {384, title = {A novel reverse phase high-performance liquid chromatography method for standardization of Orthosiphon stamineus leaf extracts}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {December 2014}, pages = {23-31}, type = {Original Article}, chapter = {23}, abstract = {

Background: Orthosiphon stamineus Benth. (Lamiaceae) is a traditional medicinal plant which has been used in treating various ailments such as kidney diseases, bladder inflammation, arthritis and diabetes. The leaves contain high concentration of phenolic compounds, thus, rosmarinic acid (RA), 3{\textquoteright}-hydroxy-5, 6, 7, 4{\textquoteright}-tetramethoxyflavone (TMF), sinensetin (SIN) and eupatorin (EUP) were chosen as a marker compounds for standardization of various O. stamineus leaf extracts. Objectives: The aim was to develop and validate a new high-performance liquid chromatography (HPLC) method for quantification of 4 marker compounds (RA, TMF, SIN, EUP) in various O. stamineus leaf extracts. Materials and Methods: The method was developed and validated using RP-HPLC-diode-array detection at 320 nm for accuracy, precision and limits of detection and was applied for quantification of it markers in five different extracts prepared in solvents with increasing polarity, using a gradient mobile phase 0.1\% formic acid: Acetonitrile at a flow rate of 1 ml/min on reverse phase acclaim polar advantage II C18 column (3 {\textmu}m, 3 {\texttimes} 150 mm) with 18 min separation time. Results: The developed method provided satisfactory precision, and the accuracy of this method was in the range of 90.2\% to 105.5\%. All of 4 compounds showed good linearity at R2 \> 0.999. Conclusion: The developed method is a simple, cost effective with shorter run time (18 min) in comparison to previous methods (30 min) and utilization of environmental-friendly solvents system. Therefore, this method has the potential to replace currently used methods in the routine standardization work of O. stamineus extracts, raw materials and its commercial products.

}, keywords = {3{\textquoteright}-hydroxy-5, 4{\textquoteright}-tetramethoxyflavone, 6, 7, Eupatorin, Orthosiphon stamineus, Rosmarinic acid, Sinensetin.}, doi = {10.4103/0974-8490.147195}, author = {Noor Hafizoh Saidan and Abdalrahim F.A. Aisha and Mohd Shahrul Ridzuan Hamil and Amin Malik Shah Abdul Majid and Zhari Ismail} }