@article {1259, title = {LC-MS and HPTLC Profile of Crude Ethanol Extract of Plant Michelia champaca Linn.}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {490-494}, type = {Original Article}, chapter = {490}, abstract = {

Aim: The plant Michelia champaca L. plant is a very rich source of phytoconstituents with medicinal properties. The aim of the study is to analyze the chemical constituents present in the crude extract responsible for various pharmacological actions. Materials and Methods: The aerial parts of the plant Extracted with ethanol and subjected to LC-MS and HPTLC analysis by using Water Q-TOF micro coupled with 2795 HPLC and CAMAG TLC SCANNER -3. Results: LC-MS analysis showed the various peaks with low and high molecular weights indicating the presence of various phytochemicals. The compounds identified in the ethanol extract include parthenolides, quercetin, stigmasterol, sitosterol, and liriodenine and some unknown compounds by comparison with the SDBS database. HPTLC of the ethanol extract showed the Rf value of five compounds, were found to be more prominent. 0.15, 0.3, 0.45, 0.6, 0.75. identified by comparison with HPTLC - derived database. Conclusion: The compounds identified by LC-MS and HPTLC of the ethanol extract of the aerial plant Michelia champaca Linn. may be responsible for its various pharmacological activities, and hence this plant can be used in the treatment of various ailments.

}, keywords = {Chromatography, Extract, Plant, Spectroscopy}, doi = {10.5530/pres.16.3.59}, author = {Chander Mohan and Seema Devi} } @article {1315, title = {Melezitose a Potential of Bioactive Compound from Aqueous Extract of Indian Satavari: An Web Based in silico Study Utilizing GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {887-896}, type = {Original Article}, chapter = {887}, abstract = {

Background: Historical records indicate that medicinal plants and their components have been utilized for treating diverse conditions since antiquity. Asparagus racemosus, in particular, demonstrates a broad spectrum of therapeutic possibilities. Aim: This study aimed to pinpoint potential bioactive compounds found within the aqueous extract of Asparagus racemosus. Materials and Methods: Gas Chromatography employed to identify the presence of various molecules, while Mass Spectrometry and FTIR analysis validated their molecular structures. The molecule was subjected to thorough scrutiny for its potential as a drug candidate, its biological activity, and its predicted targets using in silico techniques. Results: The aqueous extract stands out as the richest reservoir of carbohydrates and steroidal alkaloids. The molecule Melezitose was found in the highest concentration (37.86\%). The identified molecule is a carbohydrate in nature. The computational analysis revealed promising drug-like properties and therapeutic potential for the investigated molecule. Conclusion: It could serve as a viable therapeutic option for addressing multiple disorders.

}, keywords = {Aqueous extract, Asparagus racemosus, Chromatography, In silico study, Mass spectroscopy, Target analysis}, doi = {10.5530/pres.16.4.101}, author = {Sandip Sen and Kalepu Swathi and Dubbasi Vishwanath and Bairam Ravindar and Mitta Chaitanya and Srinivas Cherukupally and Shapuram Tejasree and Hema Varaha Santhoshi Allu} } @article {1181, title = {Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {727-732}, type = {Original Article}, chapter = {727}, abstract = {

Background: A number of nonsteroidal anti-inflammatory medications have been shown to lessen pain and inflammation. Unfortunately, there are numerous side effects connected with their use. There are, however, medicinal plants that have anti-inflammatory therapeutic effects with little to no side effects. Momordica dioica is a herb that belongs to the Cucurbitaceae family. There are numerous phytoconstituents in it. Ursolic acid is one of them and a pentacyclic terpenoid that is found in nature and has a number of therapeutic benefits. The current study looked at Ursolic Acid{\textquoteright}s activities (UA), a secondary plant metabolite, for its anti-inflammatory properties in the Momordica dioica plant. The current study{\textquoteright}s objective was to create and validate an HPTLC method that was quick, accurate, precise, and specific for determining ursolic acid from Momordica dioica herbal extract. Methodology/Conclusion/Significance: For quick analysis of Ursolic acid determination, The High Performance Thin Layer Chromatography (HPTLC) was established and confirmed. On an aluminium HPTLC plate (60 F254) coated with precoated silica gel with formic acid, ethyl acetate, and toluene (7:3:0,1), chromatographic separation was accomplished. The detection process was carried out at 530 nm. Ursolic acid{\textquoteright}s Rf value was discovered to be 0.795\%. In the 400ng/band concentration range, linearity for ursolic acid was detected. The limits of detection and quantitation for ursolic acid were observed to be 0.04 ng/ band and 0.14 ng/band, respectively. The mean \% recovery of rosmarinic acid was (0.54). The method{\textquoteright}s specificity, robustness, linearity, precision, and accuracy have all been validated in compliance with ICH standards. The created method can be used to evaluate the regularity of ursolic acid in herbal extracts.

}, keywords = {Chromatography, Herbal extract, HPTLC, Inflammation, Methanol, momordica dioica, Rf value, Ursolic acid}, doi = {10.5530/pres.15.4.077}, author = {Jui Darbhe and Vrushali Neve and Vrushali Bhalchim and Ashwini Tonpe and Pritam Khandave and Suchita Mane and Rupali Yewale} } @article {422, title = {High Performance Thin layer Chromatography: Densitometry Method for Determination of Rubraxanthone in the Stem Bark Extract of Garcinia cowa Roxb}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {230-233}, type = {Original Article}, chapter = {230}, abstract = {

Context: Garcinia cowa is a medicinal plant widely grown in Southeast Asia and tropical countries. Various parts of this plant have been used in traditional folk medicine. The bark, latex, and root have been used as an antipyretic agent, while fruit and leaves have been used as an expectorant, for indigestion and improvement of blood circulation. Aims: This study aims to determine the concentration of rubraxanthone found in ethyl acetate extract of the stem bark of G. cowa by the high-performance thin-layer chromatography (HPTLC). Materials and Methods: HPTLC method was performed on precoated silica gel G 60 F254 plates using an HPTLC system with a developed mobile-phase system of chloroform: ethyl acetate: methanol: formic acid (86:6:3:5). A volume of 5 μL of standard and sample solutions was applied to the chromatographic plates. The plates were developed in saturated mode of twin trough chamber at room temperature. The method was validated based on linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and specificity. The spots were observed at ultraviolet 243 nm. Results: The linearity of rubraxanthone was obtained between 52.5 and 157.5 ppm/spot. The LOD and LOQ were found to be 4.03 and 13.42 ppm/spot, respectively. Conclusion: The proposed method showed good linearity, precision, accuracy, and high sensitivity. Therefore, it may be applied for the quantification of rubraxanthone in ethyl acetate extract of the stem bark of G. cowa.

}, keywords = {Chromatography, Densitometry, Garcinia cowa Roxb., High-performance thin-layer chromatography, Rubraxanthone, Validation}, doi = {10.4103/pr.pr_144_16}, author = {Dachriyanus Hamidi and Hilyatul Aulia and Meri Susanti} }