@article {360, title = {HPLC-DAD-ESI-MS/MS Characterization of Bioactive Secondary Metabolites from Strelitzia nicolai Leaf Extracts and Their Antioxidant and Anticancer Activities In vitro}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {368-378}, type = {Original Article}, chapter = {368}, abstract = {
Background: Strelitzia nicolai Regel and K{\"o}rn (Strelitziaceae) is native to Southern Africa whose phytochemistry and pharmacology were slightly investigated. Materials and Methods: In the current work, different solvent extracts of S. nicolai were screened for their chemical profiles through high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS/MS) analyses. Furthermore, their in vitro antioxidant, cytotoxic, and anticancer activities were evaluated using 2,2{\textquoteright}-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2{\textquoteright}-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) \& ferric reducing antioxidant power (FRAP) and crystal violet staining (CVS) colorimetric assays, respectively. Results: HPLC-DAD-ESI-MS/MS analyses led to the identification of nineteen and eleven phenolic compounds from the ethyl acetate and n-butanol extracts, respectively including flavonoids (e.g., quercetin 3-(2 G-rhamnosylrutinoside, quercetin, quercetin-3-O-glucoside, kaempferol-3,7-O-dirhamnoside, isorhamnetin-3-O-rutinoside and kaempferol-3-O-glucoside), phenolic acids derivatives (e.g., chlorogenic acid glycoside, protocatechuic acid-O-glucoside and caftaric acid ), chalcones (e.g., xanthoangelol), and phenylethanoids (e.g., ligstroside glucoside). Moreover, in the DPPH assay the IC50 value of the most active ethyl acetate extract was 20.49 μg/mL, relative to 2.92 μg/mL of ascorbic acid. ABTS and FRAP results reinforced the results of DPPH assay. According to the National Cancer Institute criteria, the tested extracts showed weak to moderate cytotoxic activities with IC50 values ranged from 65.23 to 451.29 μg/ mL. Furthermore, the EtOAc and n-BuOH extracts showed a noticeable anticancer activity with CVS spectroscopic readings for liver hepatocellular carcinoma growth 0.806 and 0.684 at a concentration (125 μg/mL), as well as 0.730 and 0.618 at concentration (500 μg/mL), respectively against control at 1.022. Conclusion: The obtained results reveal the high efficacy of the phenolic-rich extracts from S. nicolai as naturally occurring antioxidant and anti-tumor agents.
}, keywords = {Anticancer activity, Antioxidant activity, cytotoxicity, HPLC-DAD-ESI-MS/MS, Polyphenolics, Strelitzia nicolai}, doi = {10.4103/pr.pr_89_18}, author = {Mosad Ghareeb and Amal Saad and Wafaa Ahmed and Laila Refahy and Sami Nasr} } @article {378, title = {Crude Extracts of Marine-derived and Soil Fungi of the Genus Neosartorya Exhibit Selective Anticancer Activity by Inducing Cell Death in Colon, Breast and Skin Cancer Cell Lines}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {December 2015}, pages = {8-15}, type = {Original Article}, chapter = {8}, abstract = {Background: The crude ethyl acetate extracts of marine-derived fungi Neosartorya tsunodae KUFC 9213 (E1) and N. laciniosa KUFC 7896 (E2), and soil fungus N. fischeri KUFC 6344 (E3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines. Materials and Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was performed, after 48 h treatments with different concentrations of extracts, to determine their concentration of the extract or Dox that inhibits cell viability by 50\% for each cell line. The effects of the crude extracts on DNA damage, clonogenic potential and their ability to induce cell death were also assessed. Results: E1 was found to the void of anti-proliferative effects. E2 was shown to decrease the clonogenic potential in human colorectal carcinoma cell line (HCT116), human malignant melanoma cell line (A375), human breast adenocarcinoma cell line (MCF7), and human caucasian colon adenocarcinoma Grade II cell line (HT29) cells, whereas E3 showed such effect only in HCT116 and MCF7 cells. Both extracts were found to increase DNA damage in some cell lines. E2 was found to induce cell death in HT29, HCT116, MCF7, and A375 cells while extract E3 increased cell death in MCF7 and HCT116 cell lines. Conclusion: The results reveal that E2 and E3 possess anticancer activities in human colon carcinoma, breast adenocarcinoma, and melanoma cells, validating the interest for an identification of molecular targets involved in the anticancer activity.
}, keywords = {Anti-proliferative activity, Anticancer activity, Cancer cell lines, Cell death, Marine-derived fungi, Neosartorya}, doi = {10.4103/0974-8490.171105}, author = {Alice Abreu Ramos and Bruno Castro-Carvalho and Maria Prata-Sena and Tida Dethoup and Suradet Buttachon and Anake Kijjoa and Eduardo Rocha} } @article {824, title = {Anticancer Active Homoisoflavone from the Underground Bulbs of Ledebouria hyderabadensis}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {August,2014}, pages = {303-305}, type = {Original Article}, chapter = {303}, abstract = {Background: Ledebouria is a genus of deciduous or weakly evergreen bulbs in the Hyacinthaceae family. This is recognized as the first collection made of the new taxon Ledebouria hyderabadensis, exist in the Hyderabad city of Andhra Pradesh, India. Objective: The goal of this work was to investigate the phytochemical constituents present in the new specifies and also to evaluate the cytotoxic properties of the extracts and pure compounds against human cancer cell lines. Materials and Methods: The anticancer activity was evaluated in in vitro mode by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: Phytochemical investigation of underground bulbs of indigenous, rare, and recently identified herb L. hyderabadensis yielded a bioactive homoisoflavanone, Scillascillin 1. The structure of the compound was established on the basis of various nuclear magnetic resonance and mass spectral data. The compound Scillascillin was isolated for the first time from L. hyderabadensis. In vitro anticancer activity, performed using MTT assay, showed compound 1 as significantly active against human cancer cell lines MCF-7 (breast cancer) and DU-145 (prostate cancer) with inhibitory concentration (IC) 50 values 9.59 and 11.32 μg/ml respectively when compared with herb methanol extract (IC 50 values 36.21 and 44.86 μg/ml respectively).
}, keywords = {Anticancer activity, Hyacinthaceae, Ledebouria hyderabadensis, Scillascillin}, doi = {10.4103/0974-8490.138272}, author = {Yakaiah Chinthala and Srinivas Chinde and Arigari Niranjana kumar and K.V.N. Satya Srinivas and Jonnala Kotesh Kumar and Kakaraparthy Pandu Sastry and Paramjit Grover and M Venkat Ramana} }