@article {357, title = {Hepatoprotective, Antihyperlipidemic, and Anti-inflammatory Activity of Moringa oleifera in Diabetic-induced Damage in Male Wistar Rats}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {April 2017}, pages = {182-187}, type = {Original Article}, chapter = {182}, abstract = {

Background: The number of individuals with diabetes is increasing daily, and diabetes is presently estimated to affect about 422 million adults worldwide. Conventional drugs used to treat diabetes are not without severe side effects, accessibility, and affordability. This study elucidates the potential effects of Moringa oleifera (MO) leaves extract to manage and treat diabetes induced in male Wistar rats. Materials and Methods: Adult male Wistar rats were randomly divided into four groups (n = 12/group): NC {\textendash} nondiabetic rats (positive control), MO {\textendash} nondiabetic-treated rats, DM {\textendash} diabetic rats (negative control), DM + MO {\textendash} diabetic-treated rats. Hepatic enzymes and biochemical parameters as well as antioxidant capacity and inflammatory cytokine levels were assessed. Levels of low-density lipoprotein, high-density lipoprotein, and total cholesterol were evaluated. Results: Oral administration of methanolic extract of MO (250 mg/kg) to diabetic rats for 42 days showed a significant reduction in hepatic enzyme markers and normalized lipid profile parameters in the serum compared to normal control group. Treatment also increased the level of antioxidant capacity and alleviated inflammatory biomarkers of the liver. Histology sections of the liver tissue showed protective effect of MO in treated rats. Conclusions: MO showed hepatoprotective, anti-inflammatory, and lipid-lowering effects against streptozotocin-induced hepatotoxicity. Histological section demonstrated specific alterations in the liver of the diabetic and nondiabetic male Wistar rats while MO treatment revealed improvement in liver alterations.

}, keywords = {Diabetes, Hepatic enzymes, Inflammatory cytokines, Lipid profile, Moringa oleifera, Streptozotocin}, doi = {10.4103/0974-8490.204651}, author = {Elizabeth I Omodanisi and Yapo G Aboua and Novel N Chegou and Oluwafemi O Oguntibeju} } @article {682, title = {Moringa Oleifera Lam.: Protease Activity Against Blood Coagulation Cascade}, journal = {Pharmacognosy Research}, volume = {4}, year = {2012}, month = {december,2011}, pages = { 44-49}, type = {Original Article}, chapter = { 44}, abstract = {

Background: The present study evaluated the protease activity of aqueous extracts of Moringa oleifera (Moringaceae) leaf (MOL) and root (MOR). Materials and Methods: Protease activity was assayed using casein, human plasma clot and human fibrinogen as substrates. Results: Caseinolytic activity of MOL was significantly higher (P <= 0.05) than that of MOR. Similar observations were found in case of human plasma clot hydrolyzing activity, wherein MOL caused significantly higher (P <= 0.05) plasma clot hydrolysis than MOR. Zymographic techniques were used to detect proteolytic enzymes following electrophoretic separation in gels. Further, both the extracts exhibited significant procoagulant activity as reflected by a significant decrease (P <= 0.05) in recalcification time, accompanied by fibrinogenolytic and fibrinolytic activities; clotting time was decreased from 180 {\textpm} 10 sec to 119 {\textpm} 8 sec and 143 {\textpm} 10 sec by MOL and MOR, respectively, at a concentration of 2.5 mg/mL. Fibrinogenolytic (human fibrinogen) and fibrinolytic activity (human plasma clot) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), plate method and colorimetric method. Zymographic profile indicated that both the extracts exerted their procoagulant activity by selectively hydrolyzing Aa and Bb subunits of fibrinogen to form fibrin clot, thereby exhibiting fibrinogenolytic activity. However, prolonged incubation resulted in degradation of the formed fibrin clot, suggesting fibrinolytic like activity. Conclusions: These findings support the traditional usage of M. oleifera extracts for wound healing.

}, keywords = {Caseinolytic, Fibrinogenolytic, Fibrinolytic, Moringa oleifera, Procoagulant}, doi = {10.4103/0974-8490.91034}, author = {A Satish and Sudha Sairam and Faiyaz Ahmed and Asna Urooj} }