@article {522, title = {Antihyperglycemic and Insulin Secretagogue Activities of Abrus precatorius Leaf Extract}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {303-308}, type = {Original Article}, chapter = {303}, abstract = {

Aim: Abrus precatorius leaves methanolic extract (APME) was evaluated for in vivo antihyperglycemic activity and in vitro insulinotropic effect. Materials and Methods: In vivo antihyperglycemic and insulin secretagogue activities were assessed in streptozotocin-induced diabetic rats by oral administration of APME (200 mg/kg body weight [bw]) for 28 days. In vitro insulin secretion mechanisms were studied using mouse insulinoma beta cells (MIN6-β). In vivo body weight and blood glucose and in vivo and in vitro insulin levels were estimated. Results: In diabetic rats, APME treatment significantly restored body weight (26.39\%), blood glucose (32.39\%), and insulin levels (73.95\%) in comparison to diabetic control rats. In MIN6-β cells, APME potentiated insulin secretion in a dependent manner of glucose (3{\textendash}16.7 mM) and extract (5{\textendash}500 μg/mL) concentration. Insulin secretagogue effect was demonstrated in the presence of 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium, and K+ depolarized media. Insulin release was reduced in the presence of nifedipine, ethylene glycol tetra acetic acid (calcium blocking agents), and diazoxide (potassium channel opener). Conclusion: The study suggests that APME antihyperglycemic activity might involve the insulin secretagogue effect by pancreatic beta cells physiological pathways via K+-ATP channel dependent and independently, along with an effect on Ca2+ channels.

}, keywords = {Calcium, Diabetes, Glibenclamide, insulin secretagogue, K+-ATP channel, mouse insulinoma beta cells}, doi = {10.4103/0974-8490.188881}, author = {Balekari Umamahesh and Ciddi Veeresham} }