@article {177, title = {Determination of Flavan-3-ols by High-Performance Liquid Chromatography-Photodiode Array, Antioxidant Potential, Antimicrobial Activity and Pharmacognostic Evaluation of Maytenus obtusifolia (Mart.) Leaves}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {August 2020}, pages = {313-321}, type = {Original Article}, chapter = {313}, abstract = {

Background: Maytenus obtusifolia Mart. (Syn. Monteverdia obtusifolia Mart. Biral), Celastraceae, is a species located on the coast of Brazil, which is commonly used as anti-inflammatory and on the treatment of gastric ulcers. Objectives: The aim of this study consists of the determination of quality control parameters of the plant material, as well as to develop, validate high-performance liquid chromatography-photodiode array methodology for the quantification of its analytical markers and evaluate the antioxidant potential and antimicrobial activity of the extract. Materials and Methods: The plant material and ethanolic crude extract (ECE) were evaluated through pharmacopoeial and nonpharmacopoeial methods. Anti-oxidant assay was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Minimum inhibitory concentration was used to evaluate antimicrobial activity assay. Results: The assays have provided the following results: granulometry (d50 0.82 mm), moisture content (6.41\%{\textendash}9.03\%), pH of aqueous solution (5.41), apparent bulk density (0.41 mg/mL), heavy metals (\<10 ppm), total ash (5.20\%), and sulfated ash content (6.36\%). Total flavonoid (2.14\%; 6.24\%), tannin (6.51\%; 17.85\%), and phenolic content (41.95; 134.76 mg AG/g) regarding leaves powder and its ECE, respectively. The validated method was specific, linear, precise, accurate, and robust. The content of (-)-Epigallocatechin, (+)-Catechin and (-)-Epicatechin on the ECE was 1.00\%, 0.40\%, and 0.33\%, respectively. The anti-oxidant activity was evaluated by DPPH assay and have presented an IC50 at 18.01 μg/mL. In addition, the most important antimicrobial activity of the ECE was against Aspergillus flavus (MIC at 128 μg/mL). Conclusion: These data have demonstrated to be very useful toward the analysis and pharmacognostic quality control of M. obtusifolia Mart.

}, keywords = {Bom-nome, Flavan-3-ols, Maytenus obtusifolia, Pharmacognostic evaluation, Quality control}, doi = {10.4103/pr.pr_9_20}, author = {Diego Igor Alves Fernandes de Ara{\'u}jo and Anny Palloma de Lima Arruda Fernandes and Anderson Angel Vieira Pinheiro and Augusto Lopes Souto and Kaio Arag{\~a}o Sales and Priscylla Rayama Alves Fernandes de Ara{\'u}jo and Rodrigo Silva de Andrade and Marcelo Sobral da Silva} }