@article {1453, title = {Fabrication of Snail Mucin-Silk Fibroin Hydrogel Scaffold for Guided Tissue Regeneration: A Zebrafish Fin Model Study}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {927-933}, type = {Original Article}, chapter = {927}, abstract = {

Background: Scaffolds play a pivotal role in guiding tissue regeneration by providing a suitable environment for cell growth, migration, and differentiation. Among various materials, biopolymers such as silk fibroin and snail mucin have garnered attention due to their biocompatibility, natural origin, and potential to promote cellular activities. Objectives: In this study, we explore the fabrication of a novel Snail Mucin-Silk Fibroin hydrogel scaffold designed to enhance tissue regeneration. Materials and Methods: The hydrogel scaffold has been prepared by mixing 28\% w/v gelatin, 21\% w/v PVA in distilled water and 10\% HPMC, and heated to 40{\textordmasculine}C followed by addition of a snail mucin and silk fibroin at a lower temperature and freeze dried at 4{\textordmasculine}C for 12 hr. Results: The characterization tests showed the scaffold{\textquoteright}s favourable biological properties and morphology, such as biocompatibility, hemocompatibility, ability to retain moisture, and promotion of cellular activities essential for tissue repair. Histopathological analysis showed that the snail mucin-silk fibroin hydrogel scaffold significantly enhanced fin regeneration compared to the control groups. Conclusion: Thus, the snail mucin hydrogel used in this study showed better soft tissue regeneration potential and hence be used for various applications in regenerative medicine.

}, keywords = {Hydrogel, Regeneration, Silk Fibroin, Snail Mucin, Zebra Fish}, doi = {10.5530/pres.20252176}, author = {Sofia Sebastian and Bargavi Purushothaman and Pratibha Ramani} } @article {1515, title = {Isolation of Bioactive Metabolites from Tephrosia maxima and Tephrosia callophylla: A Phytochemical and Functional Study}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {August 2025}, pages = {1341-1347}, type = {Original Article}, chapter = {1341}, abstract = {

Tephrosia maxima and Tephrosia callophylla, two lesser-studied members of the Fabaceae family, were evaluated for their phytochemical, pharmacological, and toxicological profiles in this study. Aqueous extracts from shade-dried leaves were subjected to SEM analysis, hemocompatibility testing, and assays for anti-bacterial, anti-cancer, anti-inflammatory, antioxidant, and general toxicity. SEM revealed irregular crystalline morphologies, indicative of diverse bioactive constituents. Both extracts exhibited \<2\% hemolysis, confirming systemic biocompatibility. Antibacterial activity against E. coli and S. aureus was evident, along with potent cytotoxicity against MCF-7 breast cancer cells. Anti-inflammatory and antioxidant assays demonstrated dose-dependent efficacy, with up to 80\% nitric oxide inhibition and 90\% DPPH radical scavenging. Toxicity studies using brine shrimp and zebrafish embryos showed high viability and minimal adverse effects. These findings highlight the therapeutic promise and biosafety of T. maxima and T. callophylla, supporting their potential for drug discovery and biomedical applications. Further studies are needed to isolate active compounds and elucidate molecular mechanisms.

}, keywords = {Anti-cancer, Anti-microbial, Photochemical, Tephrosia}, doi = {10.5530/pres.20250024}, author = {Anoop Kumar and Abilasha Ramasubramanian and Priyadharshini Muthumanickam and Bargavi Purushothaman} }