@article {1460, title = {Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {973-982}, type = {Original Article}, chapter = {973}, abstract = {

Background: Chitin is widely preferred in biomedical application due to its biocompatible and biodegradable nature. Naturally occurring chitin is a potential antimicrobial agent and plays a major role in degrading outer shell wall of the lower crustaceans. Naturally occurring sources of chitin are more suitable for oncological research. Objectives: In the present study, the chitin derivative isolated from marine source (Artemia franciscana cysts) is evaluated for its potential to act as a therapeutic target for colorectal adenocarcinoma. Materials and Methods: The FTIR and X-ray diffraction analysis of the isolated chitin has confirmed the presence of characteristic functional groups such as amide groups, hydroxyl groups and glycosidic linkages along with their material{\textquoteright}s crystalline structure. Results: The anticancer activity of extracted chitin derivative showed significant cytotoxic effect in this study which could be attributed to their positively charged functional groups. Further mRNA gene expression analysis has revealed that the chitin derivative also exhibited apoptotic potential against HT29 cell lines by upregulating caspase-3 and caspase-9 gene and by down regulation of B cell lymphoma-2 (Bcl-2) gene expressions. Conclusion: In conclusion the present study showed that the Artemia sp. cyst-mediated chitin derivative could be a potential anticancer agent against colorectal adenocarcinoma through their anti-proliferative activity and by activation of apoptotic pathway in HT29 cell lines

}, keywords = {Anti-cancer, Artemia, Chitin, Cyst shell, HT26}, doi = {10.5530/pres.20252138}, author = {Rajendran Poovazhagi and Subramani Thirunavukkarasu and Jeferson Stanley Santhosh and Kasi Lakshmanan and Anand Javee and Cecileya Jasmin Meshak Dhanashekaran and Narayanaswamy Radhakrishnan and Venugopal Vinod Prabhu and Kunnathur Murugesan Sakthivel and Rajan Radha Rasmi and Jiang-Shiou Hwang} } @article {1449, title = {Phytochemical Profiling, Antioxidant and Antimicrobial Activities of a Traditional Polyherbal Formulation Containing Citrus limon, Bergera koenigii, Citrus medica and Andrographis paniculata}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {881-895}, type = {Original Article}, chapter = {881}, abstract = {

Background: Polyherbal formulations combining medicinal plants are widely studied for their enhanced therapeutic potential. Materials and Methods: This study evaluates a polyherbal formulation containing leaves of Citrus limon, Bergera koenigii, Citrus medica, and Andrographis paniculata for its antimicrobic and antioxidative properties. An ethanolic extract was examined to determine its phytochemical composition, which revealed a rich existence of bioactive substances like bioflavonoids, alkali-like compounds, phenolics, and Steroidal glycoalkaloids. Results: In vitro Total Antioxidant Capacity (TAC), including DPPH radical, FRAP and scavenging of nitric oxide, exhibited significant radical-neutralizing capacities comparable to standard antioxidants. The formulation also exhibited potent antibacterial action against strains of fungi and bacteria, both gram-positive and gram-negative, suggesting a synergistic enhancement of efficacy due to the combination of plant extracts. Conclusion: The outcomes highlight the prospect of this polyherbal extract as a natural therapeutic agent to treat microbial infections and oxidative stress. The future studies should focus upon in vivo evaluations and elucidating molecular mechanisms to validate these promising results.

}, keywords = {Natural therapeutics, Oxidative stress, Phytochemicals, Polyherbal formulation}, doi = {10.5530/pres.20252131}, author = {Muthuswamy Gnanadeepan and Kunnathur Murugesan Sakthivel and Rajan Radha Rasmi and Venkatraman Thirumalai and Kumar Pavithra and Kannikaparameswari Nachimuthu} }