@article {1397, title = {Bioactive Compounds in Inocutis levis and their Cytotoxic Effects on MCF-7 and HT-29 Cancer Cells}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {608-618}, type = {Original Article}, chapter = {608}, abstract = {

Background and Objectives: Fungi are undeniable sources of all types of secondary metabolites and bioactive compounds. Inocutis levis is a polypore fungus belonging to Hymenochaetales of basidiomycetes. This study aimed to identify the compounds in the extract and investigate the cytotoxicity effect of Inocutis levis on different cell lines (MCF-7, HT-29 and HFF). Materials and Methods: The extract was obtained by rotary evaporator and LC/MS was used to identify the compounds in the extract. The cytotoxic effect of Inocutis levis against MCF-7, HT-29 and HFF cells was evaluated using MTT assay, 24 and 48 hr after the addition of the extract. Also, flow cytometry analysis was carried out to study the cell cycle arrest and apoptosis.Results: The LC/ MS assay data showed that the mushroom extract contains antioxidant compounds. Based on the MTT test, Inocutis levis extract did not decrease the viability of non-cancerous HFF cells. Still, it was able to inhibit the growth and increase the death in HT-29 cells at a concentration of 100 μg/mL after 24 and 48 hr (p\<0.05) also, MCF-7 cells at concentrations of 1 and 10 and 100 μg/ mL after 24 and 48 hr (p\<0.05). Evaluation of the cell cycle 48 hr after adding the extract by flow cytometry, showed that the mushroom extract induced apoptosis in MCF-7 cells. Conclusion: Inocutis levis contains antioxidant compounds and can inhibit the growth of HT-29 and especially MCF-7 cancer cells via the induction of apoptosis.

}, keywords = {anticancer, Apoptosis, Cell cycle, HT-29, Inocutis levis, LC/MS, MCF-7}, doi = {10.5530/pres.20252069}, author = {Fereshteh Mir Mohammad Rezaei and Fatemeh Tavakoli and Maryam Kiaei and Vahid Ramezani and Amin Zarezadeh and Masoumeh Ghobad-Nejhad} } @article {1447, title = {Fumaria parviflora Lam. Aqueous Extract Versus Simethicone in the Treatment of Infantile Colic: A Prospective Randomized Clinical Trial}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {867-872}, type = {Original Article}, chapter = {867}, abstract = {

Background: infantile colic observes 10 to 40\% of infants during first few months of life. Objectives: The purpose of the present study was to evaluate the effectiveness of Fumaria parviflora Lam. (will fumitory) aqueous extract on infantile colic. Materials and Methods: A total of 52 breastfed infants suffering from infantile colic were randomly assigned to two groups. Will fumitory group and simethicone group received will fumitory extract and simethicone for 10 days, respectively. During the period of study, changing in infants{\textquoteright} colic symptoms including duration of crying, stooling frequency and consistency as well as probable side effects were inquired every day from the parents. Results: The reduction in daily crying duration (\<50\%) was significantly in will fumitory group (25 vs. 6; p\<0.001) as well as cure rate of 100\% in this group. There were no significant differences in weight gain, stooling frequency, incidence of constipation, diarrhea and regurgitation between two groups (p\>0.05). No adverse effects related to will fumitory and simethicone group were observed. Conclusion: Will fumitory make a considerable improvement in colic related crying in comparison with simethicone. It was also well tolerable by infants suggesting as a new therapeutic approach in infantile colic.

}, keywords = {Fumaria parviflora Lam., Infantile Colic, Simethicone, Will fumitory}, doi = {10.5530/pres.20251909}, author = {Vahid Ramezani and Mahmood Noori Shadkam and Mobina Ghiasirad and Sayedeh Ebrahimi and Ali Mohammad Ranjbar} }