@article {159, title = {Screening of Cytotoxic Activity of Hematite (α-Fe2O3) Nanoparticles from Butea monosperma on MCF-7 Cells}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {August 2020}, pages = {260-266}, type = {Original Article}, chapter = {260}, abstract = {

Background: Hematite (α-Fe2O3) has been used as an antimicrobial and disinfectant agent. Nevertheless, there are limited data about antitumor potential. This study was focused on investigating cytotoxic effects of Hematite (α-Fe2O3) from Butea monosperma flower extract on MCF -7 breast cancer cells and its mechanism of action. Materials and Methods: Thus, a green method was created for the synthesis of Hematite (α-Fe2O3) using an aqueous extract of B. monosperma flower. Synthesis of Hematite (α-Fe2O3) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2{\textquoteright},7{\textquoteright}-dichlorofluorescein diacetate and intracellular calcium (Cai2+) was evaluated with probe flu3-AM. Cells were treated with different concentrations of Hematite (α-Fe2O3) (1, 3, 6, 10, 15, 25, 50, and 100 μg/mL). Results: The results showed that Hematite (α-Fe2O3) hindered cell growth in a dose-dependent manner. Hematite (α-Fe2O3) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai2+ (half-maximal inhibitory concentration 52 {\textpm} 3.14). Conclusion: In conclusion, the results of this preliminary study demonstrated that Hematite (α-Fe2O3) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.

}, keywords = {Butea monosperma, cytotoxicity, Hematite (α-Fe2O3), MCF-7 cell line, Nanoparticle}, doi = {10.4103/pr.pr_115_19}, author = {Debasish Pradhan and Shaktiprasad Pradhan and Bandana Behera and Adyasa Samantaray} }