@article {307, title = {Estimation and Comparison of Amount of Organic Acids from Dried Leaves of Garcinia cambogia, Garcinia indica, Garcinia xanthochymus, and Garcinia morella by High-Performance Liquid Chromatography}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, pages = {86-91}, chapter = {86}, abstract = {

Background: Pharmacological properties of (-)-hydroxycitric acid (HCA) derived from plants are extensively investigated for preventing lipid storage and controlling appetite. The Garcinia species is most sought out species to recover (-)-HCA. Objectives: Organic acids from dried leaves of Garcinia cambogia, Garcinia indica, Garcinia xanthochymus, and Garcinia morella were extracted and analyzed by high-performance liquid chromatography. Materials and Methods: Oven-dried leaves of the four plant samples were subjected to Soxhlet water extraction and further acid extraction. After evaporation, the residue was mixed with 50 ml, 30\% orthophosphoric acid. 20 μL of the sample was injected to a reversed-phase C 18 column under gradient elution solvent containing 0.2 M sodium sulfate with a pH of 2.5 adjusted with dilute H2SO4. At a flow rate of 0.5 mL/min, reading was taken using ultraviolet detection at 215 nm. Chromatograms of (-)-HCA, lactone, and citric acid were obtained for standards and the samples. Results: The amount of (-)-HCA, lactone, and citric acid in the dry leaves of G. cambogia was estimated at 7.95\%w/w, 3.25\%w/w, and 0.13\%w/w, respectively. In G. indica, the components were estimated at 5.71\%w/w, 3.21\%w/w, and 0.07\%w/w, respectively. In the dry leaves of G. xanthochymus, it was estimated at 0.02\%w/w, 0.06\%w/w, and 0.18\%w/w. In G. morella, it was estimated at 0.0\%w/w, 0.01\%w/w, and 0.29\%w/w. G. cambogia and G. indica have high amounts of (-)-HCA and lactone in samples of dried leaves. (-)-HCA and lactone are absent in G. morella. Higher amounts of citric acid were found in the leaves of G. morella.

}, keywords = {Citric acid, Garcinia, High-performance liquid chromatography, Hydroxycitric acid, Lactone, organic acids.}, doi = {10.4103/pr.pr_159_18}, author = {Madappa Machamada Bheemaiah and Bopaiah Ajjikuttira Kushalappa} } @article {147, title = {Estimation and Comparison of Amount of Organic Acids from Dried Leaves of Garcinia cambogia, Garcinia indica, Garcinia xanthochymus, and Garcinia morella by High-Performance Liquid Chromatography}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February 2019}, pages = {86-91}, type = {Original Article}, chapter = {86}, abstract = {

Background: Pharmacological properties of (-)-hydroxycitric acid (HCA) derived from plants are extensively investigated for preventing lipid storage and controlling appetite. The Garcinia species is most sought out species to recover (-)-HCA. Objectives: Organic acids from dried leaves of Garcinia cambogia, Garcinia indica, Garcinia xanthochymus, and Garcinia morella were extracted and analyzed by high-performance liquid chromatography. Materials and Methods: Oven-dried leaves of the four plant samples were subjected to Soxhlet water extraction and further acid extraction. After evaporation, the residue was mixed with 50 ml, 30\% orthophosphoric acid. 20 μL of the sample was injected to a reversed-phase C18 column under gradient elution solvent containing 0.2 M sodium sulfate with a pH of 2.5 adjusted with dilute H2SO4. At a flow rate of 0.5 mL/min, reading was taken using ultraviolet detection at 215 nm. Chromatograms of (-)-HCA, lactone, and citric acid were obtained for standards and the samples. Results: The amount of (-)-HCA, lactone, and citric acid in the dry leaves of G. cambogia was estimated at 7.95\%w/w, 3.25\%w/w, and 0.13\%w/w, respectively. In G. indica, the components were estimated at 5.71\%w/w, 3.21\%w/w, and 0.07\%w/w, respectively. In the dry leaves of G. xanthochymus, it was estimated at 0.02\%w/w, 0.06\%w/w, and 0.18\%w/w. In G. morella, it was estimated at 0.0\%w/w, 0.01\%w/w, and 0.29\%w/w. G. cambogia and G. indica have high amounts of (-)-HCA and lactone in samples of dried leaves. (-)-HCA and lactone are absent in G. morella. Higher amounts of citric acid were found in the leaves of G. morella.

}, keywords = {Citric acid, Garcinia, High-performance liquid chromatography, Hydroxycitric acid, Lactone, Organic acids}, doi = {10.4103/pr.pr_159_18}, author = {Madappa Machamada Bheemaiah and Bopaiah Ajjikuttira Kushalappa} }