@article {1272, title = {Assessing the Antiurolithiatic Potentials of Calamus floribundus Griff. An in vitro and in vivo Approaches}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {604-615}, type = {Original Article}, chapter = {604}, abstract = {

Background: Urolithiasis, which is acquiring clinical relevance, is one of the most prevalent diseases seen in urology clinics. The most often reported uroliths in humans are oxalate, struvite, urate, brushite and cystine. The globally rising occurrence and severity of urolithiasis has made it an issue of demanding medical attention. Medicinal herbs and natural substances have been shown to be useful in the treatment and management of urolithiasis across various recent investigations. Calamus floribundus Griff. belongs to the Aracaceae family and its urolithiatic potential has yet to be established. Objectives: The objective of the study is to evaluate the antiurolithiatic potential of methanolic extract Calamus floribundus Griff. utilizing in vitro and in vivo methods. Materials and Methods: Nucleation assay and aggregation assay was done to evaluate the in vitro antiurolithiatic activity. In the in vivo studies ethylene glycol induced and glycolic acid induced model were used. MTT assay was done to evaluate the cytotoxic effect of the methanolic extract of Calamus floribundus Griff. Results: The IC50 value of the extract was found to be 767.394{\textpm}0.22 μg/mL and 759.435{\textpm}0.29 μg/mL in the nucleation and aggregation assay respectively. In the in vivo study all the biochemical parameters were normalised in dose dependent manner. Methanolic extract exhibited cytotoxic effect on NRK-52E cell lines with a IC50 value of 11.1 μg/mL. Conclusion: The obtained results indicates for the antiurolithiatic potential of Calamus floribundus Griff.

}, keywords = {Calamus floribundus Griff, Kidney stone, Nephrolithiasis, Urolithiasis}, doi = {10.5530/pres.16.3.72}, author = {Kangkan Deka and Bibhuti Bhusan Kakoti and Ngurzampuii Sailo and Zonunmawii and Dev Jyoti Kalita and Rajashri Bezbaruah} } @article {1176, title = {Piper pedicellatum C. DC. Leaf Methanol Extract Inhibits Edema and Granuloma and Modulates Mediators and Cytokines in Rats}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {679-686}, type = {Original Article}, chapter = {679}, abstract = {

Background: Piper pedicellatum is an ethnomedicinal plant used in various inflammatory conditions by many ethnic groups and exploration of its therapeutic potentials is imperative. Objective: This study was carried out to assess anti-inflammatory potential of the methanol extract of the plant leaves using in vivo experiments to validate the traditional uses. Materials and Methods: Carrageenan-induced paw edema and Cotton pellet-induced granuloma models were used to investigate into the anti-inflammatory potential of the plant extract in rats at 200 mg/kg and 400 mg/kg dose. Indomethacin (10 mg/kg) is used as reference standard. Serum levels of inflammatory markers and cytokines, hematological and serum hepatic enzyme indices were analyzed in granuloma-induced model. Results: The methanol extract suppressed Carrageenan-induced paw edema significantly at different time intervals. The highest percentage inhibition was observed at 3rd hr after Carrageenan injection, which was 42.67\% and 49.33\% for 200 mg/kg and 400 mg/kg dose of the extract respectively. In granuloma-induced model, the methanol extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 28.58\% and 41.27\%, respectively and inhibition of granuloma tissue was 32.45\% and 43.71\%, respectively along with significant decrement in serum C-reactive protein, Prostaglandin E2, Tumor necrosis factor-α, Interleukin-6 while increases in Interleukin-10 levels. Also, the results showed hematological and serum hepatic enzyme levels were rectified to near normal levels in treated rats. Conclusion: The outcome of the investigation reveals the potential of extract as possible anti-inflammatory agent, which can be develop into therapeutic agent or supplement and also necessitates further pharmacological studies on the plant.

}, keywords = {Cytokines, Edema, Granuloma, Interleukins, Piper pedicellatum C. DC}, doi = {10.5530/pres.15.4.072}, author = {Dev Jyoti Kalita and Kangkan Deka and Ngurzampuii Sailo and Sanjoy Das and Bibhuti Bhusan Kakoti} }