@article {182, title = {Chromatographic Profiles, Anti-inflammatory, and Cytotoxicity Potential of Extracts of Banisteriopsis pubipetala (A. Juss)}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {November,2019}, pages = {339-345}, type = {Original Article}, chapter = {339}, abstract = {

Background: Banisteriopsis pubipetala is a little-studied species belonging to the same genus of B. caapi, which stands out for the bioactive compounds known to be important in the degenerative diseases treatment. Objective: The objective is to analyze the anti-inflammatory and cytotoxic potential and chromatographic profile of the extracts of B. pubipetala. Materials and Methods: The investigation of the chromatographic profile was performed through high-performance liquid chromatography diode-array detector. Cell viability was determined by a quantitative colorimetric assay with 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide. In the pharmacological tests, interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), IL-10, and nitric oxide (NO) levels in cell culture supernatants were performed. Results: In the leaf were observed nine major compounds and in the stem five major compounds. The extracts of B. pubipetala demonstrated concentration-dependent behavior regarding cytotoxicity. The fraction in dichloromethane had inhibitory concentration of 50\% (IC50) = 67.39 μg/mL whereas the extract in ethyl acetate had IC50 = 103.37 μg/mL. The results showed that extracts significantly reduced the production of IL-6 and TNF-α by cells 3T3 cells, however increased the production of IL-10 and NO. Conclusion: The results of the tests indicate that the extracts of B. pubipetala evaluated have potential anti-inflammatory properties and may promote the regulation of inflammation levels.

}, keywords = {Banisteriopsis pubipetala, Interleukin-10, Interleukin-6, Malpighiaceae, Nitric oxide, Tumor necrosis factor-alpha.}, doi = {10.4103/pr.pr_71_19}, author = {Jeane Ferreira Leal de Freitas and Fl{\'a}via Dayrell Fran{\c c}a and M{\'\i}riam Martins Chaves and Elytania Veiga Menezes and Afr{\^a}nio Farias de Melo J{\'u}nior and Dario Alves de Oliveira and Clarice Avelar Almeida and Kamylla Santos Teixeira and Murilo Malveira Brand{\~a}o and Vanessa de Andrade Royo} }