@article {1376, title = {Anthelmintic Activity of Hypericum japonicum Thunb.: An in vitro and in silico Studies}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {283-294}, type = {Original Article}, chapter = {283}, abstract = {

Background: Hypericum japonicum Thunb. is a vital medicinal plant in Northeast India, traditionally used by tribal communities to treat helminth infections with leaf extracts. Objectives: The present study investigates the in vitro and in silico studies of anthelmintic activity of H. japonicum. Materials and Methods: The anthelmintic activity was tested on Paramphistomum sp. with a test dose of 5 mg/mL to see the paralysis and death times. Phytocompounds were identified using GC-MS technique. 5 key enzymes-Alkaline Phosphatase (ALP), Acid Phosphatase (ACP), malate-and lactate dehydrogenase, and acetylcholinesterases were assayed using standard protocol. Furthermore, identified compounds were studied for their binding activity with the enzymes. Results: Diethyl ether extract of H. japonicum showed the most potent anthelmintic activity against Paramphistomum sp. in the present study. GC-MS analysis identified 12 compounds in the diethyl ether extract. Of the five enzymes studied, ALP showed highest reduction (42.59\%) and the least was found in ACP (16.21\%) compared to control. Molecular docking observed strongest binding affinity between compound-2 and AchE (-6.73 kcal/mol) followed by ALP, ACP, and MDH enzymes. Conclusion: The findings suggest that Hypericum japonicum could be a potential source of anthelmintic agents, warranting further studies to elucidate its exact mechanism of action.

}, keywords = {Anthelmintic, GC-MS, Hypericum japonicum, Molecular docking, Molecular dynamics}, doi = {10.5530/pres.20252026}, author = {Mritunjoy Kumar Roy and Ananta Swargiary} } @article {1139, title = {Phytochemical, Antioxidant and Trace Element Analysis of Hypericum japonicum Thunb.}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {338-346}, type = {Original Article }, chapter = {338}, abstract = {

Background: Plants possess various bioactive compounds with many biological activities. Antioxidant plays vital roles in initiating deleterious free scavenging radical reactions. Objectives: The present study was done to determine the presence of phytochemical properties of the plant along with its antioxidant analysis of four solvent fractions (hexane, diethyl ether, ethyl acetate and methanol) from Hypericum japonicum. Furthermore, five heavy metals- Zn, Cu, Cd, Pb and Cr were analyzed. Materials and Methods: All the experiments for phytochemical and antioxidants of different solvent extracts were investigated following standard protocols. The heavy metal content was analyzed using spectroscopy method. Results: Qualitative analysis revealed that almost all the phytochemicals were present on one or other solvents. The carbohydrate and protein content was highest in hexane and ethyl acetate extract. Ethyl acetate showed highest phenol content while diethyl ether showed highest in flavonoid content. Similarly, ethyl acetate extract showed strongest antioxidant activity. Heavy metals were found in very negligible amounts. Conclusion: The present study suggests that H. japonicum might have the potential to be a good source of phytochemicals and antioxidants. Further studies are required to analyze the pharmacological properties of the plant.

}, keywords = {Antioxidant, Heavy metal, Hypericum japonicum, Medicinal plants, Phytochemical.}, doi = {10.5530/pres.15.2.036}, author = {Mritunjoy Kumar Roy and Ananta Swargiary} }