@article {1135, title = {Antidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {315-327}, type = {Original Article }, chapter = {315}, abstract = {

Background: Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse the in vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract of E. subrigida (HE{\textendash}Es) in BALB/c mice. Materials and Methods: The HE{\textendash}Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g., feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin{\textendash}3-O-β-glucoside and isorhamnetin{\textendash}3-O-β-glucoside were the main flavonoids in the HE{\textendash}Es. Glycemia was reduced by the HE{\textendash}Es (200 mg/kg b.w., 49.1\%) and glibenclamide (10 mg/kg b.w., 52\%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE{\textendash}Es (100 mg/kg b.w., 29.32\%; 200 mg/kg, 28.99\%) and acarbose (10 mg/kg b.w., 19.87\%) treatments. On the other hand, the HE{\textendash}Es was innocuous in mice (LD50 \> 1000 mg/kg b.w.), and the results suggested that HE{\textendash}Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE{\textendash}Es satellite after treatment withdrawal. Conclusion: This study reports for the first time the in vivo effects on blood glucose levels of HE{\textendash}Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE{\textendash}Es. Future studies must corroborate the effects of HE{\textendash}Es in humans, allowing its use in high-value formulations.

}, keywords = {Antihyperglycemia, Crassulaceae family, Hypoglycemia, Plant natural extract, Safety}, doi = {10.5530/pres.15.2.034}, author = {Belinda Heredia-Mercado and Francisco Delgado-Vargas and Lorenzo Ulises Osuna-Mart{\'\i}nez and Elvic Noguera-Corona and Jos{\'e} {\'A}ngel L{\'o}pez-Valenzuela and Rosalio Ramos-Pay{\'a}n and Gabriela L{\'o}pez-Angulo} }