@article {1270, title = {Antioxidant Activity of Gelatins from Sika Deer (Cervus nippon)}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {588-593}, type = {Original Article}, chapter = {588}, abstract = {

Background: In Japan, the rapid increase in the number of sika deer (Cervus nippon) has become a social issue and measures for the resource utilization of extensively captured sika deer have not kept pace. In contrast, various body parts of deer have been used in traditional Chinese medicine since ancient times. Objectives: In the present study, the in vitro antioxidant activity of gelatins from sika deer was evaluated in order to increase their usefulness as health promoting resources. Materials and Methods: Three kinds of deer gelatins were prepared through hot water extraction of antlers, skins, or bones obtained from sika deer. The antioxidant activity was evaluated along with commercially available gelatins and collagen peptides from donkey, bovine, pig and fish using three kinds of in vitro antioxidant assays including DPPH free radical scavenging activity (DPPH) assay, Superoxide anion scavenging activity (SOD) assay and Oxygen Radical Absorption Capacity (ORAC) assay. Results: All kinds of gelatins from deer exhibited more than 50\% inhibition of DPPH free radical and superoxide anion scavenging activities, as well as H-ORAC values exceeding 8,000 μmol TE/L. The gelatins from deer were further separated into high molecular-weight and low molecular-weight fractions by ethanol precipitation method and the low molecular-weight fractions showed stronger antioxidant activity than corresponding high molecular-weight fractions in all measurements. Conclusion: The result of present study provided rudimentary evidence for the antioxidant activity of gelatins from sika deer, which need further research for the use as health promoting resources.

}, keywords = {Antioxidant activity, Collagen peptide, Gelatin, Sika deer}, doi = {10.5530/pres.16.3.70}, author = {Kouharu Otsuki and Shizuka Uchino and Dongxia Li and Takashi Kikuchi and Ken Tei and Wei Li} } @article {1115, title = {Simultaneous Quantitative Determination of Six Triterpenoid Saponins in Ardisia japonica Collected from Different Regions of China through LC-ESI-MS}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {December 2022}, pages = {138-144}, type = {Original Article}, chapter = {138}, abstract = {

Background: The whole plants of Ardisia japonica (Myrsinaceae) are used in traditional Chinese medicine for the treatment of phlegm, cough, jaundice, edema, and bruise, and triterpenoid saponins are the main bioactive components. However, triterpenoid saponins from this plant have not yet been simultaneously quantitatively determined and reported. Objectives: The present study aimed to establish a fast, simple, and reliable LC-ESI-MS analysis method for simultaneous quantization of the major triterpenoid saponins in A. japonica for the first time. Materials and Methods: Six major triterpenoid saponins from the plant species of Ardisia were selected as the reference compounds. Five batches of A. japonica were gathered from five distinct regions of P. R. China. Quantitative analysis was developed and validated by LC-ESI-MS in SIM mode. The separation conditions were optimized on a RP C18 Column with ACN and 0.1\% FA (v/v) at a flow rate of 0.2 ml/min and column temperature of 35{\textdegree}C. Results: The validation investigation found that the analysis method was accurate, recoverable, and sensitive for quantification of the six triterpenoid saponins. All of the calibration curves revealed acceptable linear regression (R2\>0.9975). The contents of six triterpenoid saponins in A. japonica from five regions varied considerably. Conclusion: The present study established a highly efficient analytical technique to quantify the triterpenoid saponins in A. japonica, and it could be further applied in the quality control of A. japonica.

}, keywords = {Ardisia japonica, LC-MS, Quantitative analysis, Triterpenoid saponin}, doi = {10.5530/097484900260}, author = {Ling Ma and Mi Zhang and Kouharu Otsuki and Yan Lei and Takashi Kikuchi and Yinghua Wang and Wei Li} }