@article {263, title = {Analysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {February 2017}, pages = {34-38}, type = {Original Article}, chapter = {34}, abstract = {

Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS. Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas. Materials and Methods: Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Results: The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas. Conclusions: Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.

}, keywords = {Hierarchical cluster analysis, Natural Cordyceps sinensis, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Soluble protein, Two-dimensional electrophoresis}, doi = {10.4103/0974-8490.199782}, author = {Chun-Hong Li and Hua-Li Zuo and Qian Zhang and Feng-Qin Wang and Yuan-Jia Hu and Zheng-Ming Qian and Wen-Jia Li and Zhi-Ning Xia and Feng-Qing Yang} } @article {135, title = {Screening and Validating of 1, 1-Diphenyl-2-Trinitrophenylhydrazine Scavengers from Danshen-Honghua Herbal Pair}, journal = {Pharmacognosy Research}, volume = {11}, year = {2011}, month = {February 2019}, pages = {14-19}, type = {Original Article}, chapter = {14}, abstract = {

Background: Salvia miltiorrhiza (Danshen [DS]) and Carthamus tinctorius (Honghua [HH]) are commonly used traditional Chinese medicines for activating blood and removing stasis, and they were usually used as DS-HH (Danshen-Honghua [DH]) herbal pair in clinical applications. Characterizing the antioxidant active ingredients in DH herbal pair may be helpful for understanding their curative effect on cardiovascular diseases. Objective: The objective of the study is to screen the 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) scavenging active compounds in DH herbal pair by spectrum-effect relationship analysis. Materials and Methods: First, the water extracts of DH herbal pair with different ratios (DS: HH = 1:0, 0:1, 1:1, 2:1, 3:1, 5:1, 1:5, 1:3, and 1:2) were prepared. Then, the clearance effects of DH herbal pair and single drugs on DPPH were compared, meanwhile, high performance liquid chromatography was applied for chemical analysis of DH extracts. Finally, DPPH scavengers in DH herbal pair were predicted and identified by spectrum-effect relationship analysis and liquid chromatography-mass spectrometry (LC-MS) analysis. Results: Compared with single drugs, the extracts of herbal pairs had higher clearance rate to DPPH. Eighteen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and 13 of them were tentatively identified by LC-MS analysis. Furthermore, antioxidant activities of eight pure compounds from the DH herbal pair were validated by DPPH radical scavenging assay with Vitamin C as positive control drug. Among them, six compounds including danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin, and salvianolic acid A were found to have high antioxidant activity. Conclusion: DH herbal pair showed strong clearance effect on DPPH, and danshensu, protocatechuic acid, coffee acid, chlorogenic acid, rutin and salvianolic acid A are the active components.

}, keywords = {1, 1-diphenyl-2-trinitrophenylhydrazine scavenger, Active ingredients, Antioxidation effect, Danshen-Honghua, Spectrum-effect relationship analysis}, doi = {10.4103/pr.pr_94_18}, author = {Ya-Li Wang and Shi-Jun Yin and Sheng-Li Huang and Qian Zhang and Hua Chen and Feng-Qing Yang} }