@article {1242, title = {Evaluation of Mixer of Essential Oils and their Standard Components against Fungi Causing Superficial Mycosis in Human}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {April 2024}, pages = {339-346}, type = {Original Article}, chapter = {339}, abstract = {

Background: Dermatophytoses are responsible for almost one-quarter of all fungal infections globally. When treatable, these challenging fungal infections of the skin, hair, and nails are becoming more resistant to standard antifungal medications and frequently call for lengthy therapeutic regimens. Objectives: Essential oils have been used to cure a range of diseases for ages. In present study combination effect of volatile oils obtained from Trachyspermum ammi, Coriandrum sativum and Cymbopogon martinii were evaluated against four selected dermatophytic fungi namely Microsporum canis, M. fulvum, Trichophyton rubrum and T. mentarophytes. Materials and Methods: Four combinations like T. ammi + Cymbopogon martinii, T. ammi + Coriandrum sativum, Cymbopogon martinii + C. sativum, T. ammi + Cymbopogon martinii + C. sativum were prepared and evaluated through semisolid agar antifungal susceptibility methods. Results: During present investigation T. rubrum was found to be most resistant fungus. Combination of T. ammi with C. sativum showed excellent results as compared to T. ammi and C. martinii. Combination of C. martinii and C. sativum was less effective as compared to other combinations. MIC was ranging from 0.1 μL/mL to 0.4 μL/mL against test fungi. Conclusion: Although essential oils are frequently combined in the use of aromatherapy to cure infectious disorders, there is very little data to back up this practice. This study supports the concurrent usage of blended essential oils in some way.

}, keywords = {Combination, Dermatophytes, Essential oil, MIC.}, doi = {10.5530/pres.16.2.43}, author = {Neetu Jain} } @article {1217, title = {A Two-Year Comparative Study of the Anti-dermatophytic Activity of Thuja orientalis Essential Oil Stored at Various Temperatures}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {191-196}, type = {Original Article}, chapter = {191}, abstract = {

Background: Thuja orientalis generally recognized as Chinese arborvitae, placed under the family Cupressaceae is a commonly available medicinal plant in India. Objectives: In present research work Thuja leaves essential oil stored in three temperatures for two-years storage periods was evaluated against fungi causing ring worm infection in man. Materials and Methods: Thuja oil was hydro-distilled and stored for 24 months in airtight amber glass bottles at three distinct temperatures. MIC was determined through semi solid agar diffusion method containing BHIA medium. Anti-dermtophytic activity of stored oil was examined in triplets after every fourth month of storage up to 2 years. Results: Oil stored in refrigerator was found to be more stable up to two year of storage time. No activity changes were observed in this condition against all test fungi. While variable temperature condition of room temperature was found less suitable for the storage of oil. MIC against Candida albicans was increased 40\% after 2 years (\>3.5 μL/mL) and 66\% against Microsporum fulvum. Oil stored at 30{\textordmasculine}C temperature condition was found more stable upto IV testing. Slightly changes were observed during Vth testing against C. albicans, M. fulvum and M. canis. Conclusion: Present study concluded that essential oil of Thuja put away in chilliness condition can reserved their quality and properties of antifungal behaviour for long time. Accordingly, putting away of volatile oil ointment items in coolness (beneath 8{\textordmasculine}C) condition is best guidelines for long term use of therapeutic items.

}, keywords = {MIC., Microsporum, Thuja, Trichophyton, Volatile oil}, doi = {10.5530/pres.16.1.24}, author = {Neetu Jain and Seema Bhadauria} } @article {1058, title = {Comparative Analysis of Antidermatophytic Potential of Cymbopogon citratus Essential Oil Stored in Different Temperature Condition for Two Years}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {July 2022}, pages = {241-245}, type = {Original Article}, chapter = {241}, abstract = {

Background: Cymbopogon citratus commonly known as lemon grass is a valuable medicinal plant. In present investigation variation in the antidermatophytic potential of C. citratus essential oil was estimated in different temperature and storage periods. Materials and Methods: C. citratus essential oil was extracted through hydrodistillation methods and kept in amber bottles in three different temperature conditions like room temperature (variable from 8-45{\textdegree}C), refrigerator temperature(2-6{\textdegree}C) and 30{\textdegree}C for 2 years. Minimum inhibitory concentration was determined after every fourth month of storage against selected dermatophytes. Results: Antidermatophytic activity of C. citratus oil was found prominent due to high concentration of citral derivatives. MIC was found to be stable against Trichophyton rubrum (MTCC 296) (0.1μL/mL) and T. mentagrophytes (MTCC 7687) (0.1μL/mL) at the end of experiment in all three temperature conditions. 30{\textdegree}C temperature condition was also found quite stable. A very slight increase of MIC was found against Candida albicans (MTCC 3018) and Microsporum fulvum (MTCC 2837) at 24 months (Sixth testing) of storage. Oil stored at room temperature showed variation in MIC as compared to initial one. MIC was found to double against M. fulvum (MTCC 2837) at sixth testing (0.4μL/mL) of storage as compared to first testing (0.2μL/mL). Conclusion: Present work concluded that essential oil stored in low temperature condition can maintained their primary composition and properties for long time. Therefore, storing of essential oil products in low temperature (below 8{\textdegree}C) condition is best practices for long time application of medicinal products.

}, keywords = {C. citratus, Dermatophytes, Essential oil, Longevity, MIC, Storage}, doi = {10.5530/pres.14.3.34}, author = {Neetu Jain and Meenakshi Sharma} }