@article {1190, title = {Attenuation of Oxidative Stress and Nephrotoxicity with Supplementation of Pimpinella tirupatiensis Tuberous Root in Streptozotocin-induced Diabetic Rats}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {813-818}, type = {Original Article}, chapter = {813}, abstract = {

Background: The endemic plant of Seshachalam hills, Eastern Ghats, India, known as Pimpinella tirupatiensis (Pt). Objectives: It was examined in this study for its protective effects against oxidative damage in the kidney of Streptozotocin (STZ)-induced diabetic rats. Materials and Methods: After the induction of diabetes with Streptozotocin (40 mg kg-1 b.w), PTAq, an aqueous extract of Pt was directed orally at a dose of 750 mg kg-1 b.w. day-1. Results: Diabetic rats displayed significant decreases in Glutathione (GSH), ascorbic acid, and Vitamin E content (p\<0.01) and significant elevations in lipid peroxidation (as MDA), uric acid content, and Xanthine Oxidase (XOD) activity (p\<0.01). However, PTAq supplementation in the diabetic group resulted in significant (p\<0.01) increases in Glutathione (GSH), Ascorbic Acid (AA), and Vitamin E content and declined MDA content, uric acid content, and XOD activity. Conclusion: The results advocate that Pt has the potential to avert diabetic complications triggered by oxidative stress in experimental diabetic rats and could have therapeutic implications for diabetes management in the future.

}, keywords = {Ascorbic acid, Glutathione, Lipid peroxidation, Non-enzymatic antioxidants, Pimpinella tirupatiensis}, doi = {10.5530/pres.15.4.086}, author = {Thopireddy Lavanya and Kakarla Chandra Mohan and Kesireddy Sathyavelu Reddy and Saddala Rajeswara Reddy} } @article {1052, title = {Phytochemical Analysis of Cleome viscosa Active Polyphenolic Compounds Possessing Antidiabetic Activity}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {April 2022}, pages = {195-203}, type = {Original Article}, chapter = {195}, abstract = {

Background: The counteractive action of diabetes by plant polyphenols along with their utilization in the treatment of diabetic difficulties is of expanding significance from the epidemiological information with in vitro and in vivo examinations. Objectives: We assessed the antihyperglycemic impacts of methanolic (MeCV), aqueous (AqCV), ethyl acetate (EaCV) and n-hexane (NhCV) extracts of Cleome viscosa whole plant. Materials and Methods: Qualitative phytochemical analysis and antidiabetic activities by glucose diffusion, inhibitory movement of starch processing enzymes i.e. α-glucosidase and α-amylase, glucose uptake by yeast cell method and also the non-enzymatic action (glycosylation of Hb). The identification of polyphenols in bioactive extracts was categorized by using HPLC/UV. Results: To evaluate the comparative power of these extracts, the concentrations required for 50\% and 100\% inhibition of enzyme movement were resolved. Greatest enzyme inhibition was utilized to evaluate an inhibitor{\textquoteright}s relative viability. Results demonstrated that MeCV strongly diffused by glucose and inhibited both α-glucosidase and α-amylase enzyme movement and great impact on the glucose uptake by yeast cells and non-enzymatic glycosylation of Hb with equivalent intensity respectively than acarbose. While NhCV was showed less impact on glucose diffusion, α-glucosidase, α-amylase, glucose uptake by yeast cells and glycosylation of Hb. Gallic acid (2.45\%), protocatechuic acid hexoside (0.83\%), chlorogenic acid (0.77\%), catechin (0.53\%), quercetin (0.80\%), p-hydroxybenzoic acid (0.78\%), and p-coumaric (0.07\%) were the main compounds of the MeCV. Conclusion: Based on our results we concluded that the MeCV firmly impacts on enzymatic and non-enzymatic activities due to rich content of polyphenols present in MeCV.

Key words: Enzyme inhibition, α-Glucosidase, α-Amylase, Cleome viscosa, HPLC/UV,

}, doi = {10.5530/pres.14.2.28}, author = {Suresh Yarrappagaari and Lavanya Thopireddy and Srinivasulu Cheemanapalli and Venkata Rami Reddy Narala and Kakarla Chandra Mohan and Rajeswara Reddy Saddala} }