@article {1340, title = {Analytical Characterization of Achyranthes aspera L. Whole Plant}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {85-93}, type = {Original Article}, chapter = {85}, abstract = {

Background: The therapeutic properties of Achyranthes aspera have long been acknowledged and integrated into diverse traditional healthcare like Ayurvedic, Siddha, Unani and Naturopathic medicine for the management of various ailments. In ancient Indian systems of medicine such as Siddha and Ayurveda, the constituents found in this medicinal plant play a pivotal role in treating disorders. Both macro and micro elements are crucial components for plants to carry out their basic functions. Objectives: Consequently, the ash of Achyranthes aspera was analyzed chemically, with a focus on phytochemical and chromatographic analysis, along with spectral characterization of the entire plant, to determine the presence of inorganic constituents. Materials and Methods: Standard methods were followed for all physicochemical parameters. Chromatographic characterization was carried out using High Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC). Additionally, elemental characterization was performed using different instruments such as Energy Dispersive X-ray analysis (EDX), X-ray Fluorescence (XRF), Powder X-ray Diffraction (PXRD), X-ray Photoelectron Spectroscopy (XPS) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to investigate the minerals present in the ash of Achyranthes aspera. Results: The HPTLC photo documentation revealed the presence of five spots at 254 nm and eight spots at 366 nm. Vanillin sulfuric acid reagent derivatization resulted in the appearance of 9 spots under white light. Furthermore, the HPLC chromatogram showed the separation of 13 peaks at 366 nm. The evaluation techniques also detected various elements such as Carbon (C), Oxygen (O), Sodium (Na), Magnesium (Mg), Aluminium (Al), Silicon (Si), Phosphorus (O), sulphur (S), Chlorine (Cl), potassium (K), Calcium (Ca), Manganese (Mn), Chromium (Cr), Iron (Fe) and Zinc (Zn). Conclusion: The analysis primarily focuses on quality control criteria and the mineral composition of the plant.

}, keywords = {Achyranthes aspera, ICP-OES, Nayurivi, PXRD, SEM-EDX, XPS, XRF}, doi = {10.5530/pres.20251928}, author = {Achintya Kumar Mandal and Saravanan Meenakshisundaram and Subashini Subramanian and Sujith Thatipelli and Rajesh Chandran and Shakila Ramachandran} } @article {1026, title = {Development of Chromatographic Fingerprint Profile and Multi-elemental Analysis of Datura metel L.}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {April 2022}, pages = {146-152}, type = {Original Article}, chapter = {146}, abstract = {

Background: The Solanaceae family member Datura metel L. has been related to therapeutic benefits. The plant is widely used in Ayurveda and Siddha systems of Indian medicines. Objectives: Present study was focused to understand the phytochemical and multi elemental composition of D. metel whole plant. Materials and Methods: The whole plant of D. metel was collected, shade dried, powdered and subjected to analysis of physico-chemical parameters, thin layer chromatography, high performance thin layer chromatographic finger print profilings (HPTLC), high pressure liquid chromatographic profile at four wavelengths, X-ray fluorescence, energy dispersive X-ray analysis, X-ray photoelectron spectrometer, powder X-ray diffractometer and inductively coupled plasma optical emission spectrometer. Results: In HPTLC, 7 spots under 254 nm, 10 spots under 366 nm and 11 spots after derivatization with vanillin sulphuric acid reagent appeared. In HPLC, 9 peaks at 230 nm, 11 peaks at 254 nm, 5 peaks at 286 nm and 7 peaks at 366 nm separated. Presence of 17 elements such as copper, cobalt, nickel, manganese, zinc, iron, sodium, potassium, calcium, magnesium, phosphorus, aluminium, chlorine, carbon, oxygen, sulphur and silicon were identified. Conclusion: The elements K, Mg, Ca, Si, S and Fe play vital role on human health. Chlorine, silicon, sulfur and silica are first time identified which have significant biological functions.

Key words: Ummattai, Devil{\textquoteright}s trumpet, Solanaceae, Cardiarhythm, Asthma.

}, doi = {10.5530/pres.14.2.21}, author = {Shakila Ramachandran and Saravanan Meenatchisundaram and Subashini Subramanian and Sujith Thatipelli and Achintya Kumar Mandal and Rajesh Allu} } @article {1020, title = {Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {30-34}, type = {Original Article}, chapter = {30}, abstract = {

Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C18 column (shim-pack) of size 150 mm x 4.6 mm; 5μ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at Rt 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 μg/ml, calibration curves were produced with a linear relationship R2 \> 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2\%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 μg/ml; quantification limits were 0.473 and 0.234 μg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RPHPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

}, keywords = {3, 3{\textquoteright}, 4{\textquoteright}-pentahydroxy flavanone, 5, 7, Astilbin, Dihydroqucertin, Tuberculosis, Validation.}, doi = {10.5530/pres.14.1.6}, url = {https://phcogres.com/fulltext/v14i1/105530pres1416.html}, author = {Subashini Subramanian and Shakila Ramachandran} }