@article {1028, title = {Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {April 2022}, pages = {127-134}, type = {Original Article}, chapter = {127}, abstract = {

Objectives: This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of hydroalcoholic extract from leaves of S. marginata (EESM) in in vitro and in vivo models. Methods and Results: EESM (0.98{\textendash}1000 μg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 μg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 μg/mL while EESM did not interfere against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCGinduced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. Conclusion: This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.

Key words: Mycobacterium tuberculosis, Bacillus Calmette-Guerin (BCG), Cip{\'o}-timb{\'o}, Inflammation, Tuberculosis.

}, doi = {10.5530/pres.14.2.18}, author = {Maicon Matos Leit{\~a}o and Joyce Alencar Santos Radai and Luis Fernando Benitez Macorini and Thiago Leite Fraga and Silvia Cristina Heredia-Viira and Claudia Andrea Lima Cardoso and Arielle Cristina Arena and Candida Aparecida Leite Kassuya} } @article {126, title = {Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {April 2021}, pages = {28-33}, type = {Original Article}, chapter = {28}, abstract = {

Objectives:\ The folk use of\ Doliocarpus dentatus\ for pain and inflammatory conditions led our group to evaluate the anti-inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models.\ Results:\ Oral treatments with EEDd (100{\textendash}300 mg/kg) significantly inhibited the formalin-induced nociceptive and cold sensitivity, prevented acetic acid-induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3{\textendash}30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan-induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100{\textendash}300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3{\textendash}90 μg/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female\ Swiss\ mice or, for the CFA test, C57BL/6 mice.\ Conclusion:\ This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.

}, keywords = {Arthritis, Articular inflammation, Betulinic acid, Doliocarpus dentatus, Pain}, doi = {10.4103/pr.pr_79_20}, author = {Lidiane Schultz Branquinho and Maria Helena Verdan and Saulo Euclides Silva-Filho and Rodrigo Juliano Oliveira and Claudia Andrea Lima Cardoso and Arielle Cristina Arena and Candida Aparecida Leite Kassuya} }