@article {991, title = {Potential Cosmeceutical Applications and Evaluation of Human Skin Irritation of Tagetes erecta L. Flower Extract}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {October 2021}, pages = {199-207}, type = {Original Article}, chapter = {199}, abstract = {

Background: Tagetes erecta L. has been traditionally used for treatment of skin diseases. Objectives: This study aimed to evaluate in vitro skin anti-aging, antioxidant, antibacterial activities, and human skin irritation of T. erecta flower extract for development as a cosmeceutical skin product using aqueous, 50\%, and 95\% ethanolic extracts of T. erecta flower. Methods: Total phenolic content was defined. Quantitative analysis of syringic acid was determined by using HPLC. Antioxidant activities were performed by measurement of 2,2-diphenyl 1-pichylhydazyl free radical scavenger. Anti-aging assays were monitored inhibitory effects on tyrosinase, elastase, hyaluronidase, and collagenase activities. Antibacterial effects against Staphylococcus aureus and Staphylococcus epidermidis was also investigated. The extract was formulated as a lotion and its stability study was evaluated and human skin irritation tests were performed. Results: The 95\% ethanolic extract had significantly higher total phenolic content and syringic acid content than the other extracts. The 95\% ethanolic extract also had stronger inhibitory activities on DPPH scavenging and against tyrosinase, elastase and hyaluronidase enzyme activities than those of 50\% ethanolic or water extracts. The 95\% ethanolic extracts showed a better antibacterial activity against S. aureus and S. epidermidis. The developed lotion containing 1\% of T. erecta extract using 95\% ethanol was stable. Moreover, skin irritation was not observed in healthy volunteers after applying the T. erecta lotion. Conclusion: This study indicates that T. erecta flower extracts, especially a 95\% ethanolic extract, are potential sources for development of cosmeceutical skin products against enzymes/reactions responsible for skin aging and bacterial skin infection.

}, keywords = {Anti-aging, Asteraceae, Marigold, Skin irritation, Tagetes erecta L.}, doi = {10.5530/pres.13.4.12}, author = {Catheleeya Mekjaruskul and Supakanya Kumkarnjana and Niramai Fangkrathok and Worapol Kengkittipat and Nuttawut Sirichat} }