@article {1025, title = {Anticancer Activities of the Herbal Recipe Benjakul Extract against Human Breast Cancer Cells by Inducing Apoptosis and Suppressing Migration}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {April 2022}, pages = {115-120}, type = {Original Article}, chapter = {115}, abstract = {

Background: Benjakul is a Thai traditional formulation and has been reported for cancer treatment. Objectives: In this study aimed to examine the cytotoxic effects of Benjakul extract on suppression of breast cancer cells proliferation and migration. Materials and Methods: Cytotoxic effects were determined by sulforhodamine B, colony formation, ROS formation, caspase 3 activity, and mitochondrial function. Migration assay was studied by wound healing and gelatin zymography. Results: Piperine level was showed the highest concentration in the Benjakul extract. Further, Benjakul extract exhibited cytotoxicity against breast cancer cells with low IC50 equally effects in two cancer cells and further it suppressed the colony formation. The mechanism of Benjakul to induce cells death through activating ROS formation, stimulating caspase 3 activity, and repressing mitochondrial function. Moreover, the suppression of migration by Benjakul was acted on reduction of MMP-9 expression level in culture medium. Conclusion: Our findings demonstrated that Benjakul extract can suppress human cancer cells proliferation along with induction of apoptosis and attenuation of migration by low concentration.

Key words: Benjakul, Breast cancer cells, Growth, Apoptosis, Migration.\ 

}, doi = {10.5530/pres.14.2.16}, author = {Benjaporn Buranrat} } @article {55, title = {Cytotoxic and Antimigratory Effects on Michigan Cancer Foundation-7 Cells of Morinda citrifolia L. Leaf Extract and Formulation of Tablets from Extract}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {February 2020}, pages = {24-28}, type = {Original Article}, chapter = {24}, abstract = {

Background: Cancer is one of the deadliest diseases known to man. Efforts to combat the disease remain ongoing. Morinda citrifolia L. is a medicinal plant which is gaining interest as a natural chemotherapeutic agent for breast cancer treatment. Objective: This study aimed to determine the anticancer activity of M. citrifolia leaf extract on Michigan cancer foundation 7 (MCF-7) breast cancer cells as well as formulate tablets containing the extract to assess their viability. Materials and Methods: M. citrifolia leaf extract was prepared using the maceration method. Cytotoxicity and cell migration suppression, representing anticancer activity, were also assessed in MCF-7 cells by the sulforhodamine B assay and wound-healing assay. Tablets containing the extract were formulated using the wet granulation method. The prepared tablets were then evaluated regarding their physical properties and scopoletin content, a marker in the extract, before and after stability testing. Results: The extract showed cytotoxicity on MCF-7 cells in a dose- and time-dependent manner with 50\% inhibitory concentration values of 39.9 {\textpm} 3.5 {\textmu}g/mL for 48 h. In addition, it showed an antimigratory effect on MCF-7 cells with a significant effect at 25 {\textmu}g/mL. The prepared tablets had good characteristics and were found to meet the requirements of the United States Pharmacopeia. Moreover, they could maintain their physical properties and scopoletin content over a 2-month period. Conclusion: This study showed that M. citrifolia leaf extract exhibited potential anti-breast cancer activity. The prepared tablets containing the extract could be a potential formulation for breast cancer treatment. However, the underlying mechanism of the extract as an antibreast cancer agent requires further investigation in subsequent study.

}, keywords = {Antibreast cancer, Antimigratory effect, Morinda citrifolia L. leaf, Scopoletin content, Tablets}, doi = {10.4103/pr.pr_66_19}, author = {Supavadee Boontha and Benjaporn Buranrat and Tasana Pitaksuteepong} }