@article {124, title = {Preparation of Diterpenes-Enriched Extract from Croton stellatopilosus Ohba Leaves using Enzyme- and Ultrasonic-Assisted Extraction}, journal = {Pharmacognosy Research}, volume = {13}, year = {2021}, month = {April 2021}, pages = {22-27}, type = {Original Article}, chapter = {22}, abstract = {

Background:Croton stellatopilosus\ Ohba accumulates several diterpenes. Among them, plaunotol, a cytoprotective agent, has been prescribed to treat peptic ulcers.\ Objectives:\ We aimed to prepare the diterpene-enriched extract (DEE) using enzyme- and ultrasonic-assisted extractions.\ Materials and Methods:\ The type and amount of pectinase and cellulase and the condition of ultrasonic treatment were optimized. A combination of enzyme and ultrasonic treatments was performed. The high-performance liquid chromatography (HPLC) method was validated and used for the determination of plaunotol and plaunolide contents in the DEE.\ Results:\ A combination of cellulase (10 units) and ultrasonic (at 80\% amplitude) treatments enhanced the yield of plaunotol significantly. Application of the crude extract on diaion HP-20 resin afforded the DEE. With the validated HPLC method, the DEE contained 8.81\% {\textpm} 0.02\% w/w of plaunotol and 8.40\% {\textpm} 0.92\% w/w of plaunolide.\ Conclusion:\ We established an efficient method to prepare DEE based on green extraction. This method showed about eightfold enhancement purification factor compared to the original crude extract and about 90\% yield recovery.

}, keywords = {Cellulase-assisted, Croton stellatopilosus, Diterpenes-enriched extract, Plaunolide, Plaunotol, Ultrasonic-assisted}, doi = {10.4103/pr.pr_90_20}, author = {Wai Mi Aung and Supattra Limsuwanchote and Juraithip Wungsintaweekul} }