@article {1050, title = {Phytochemical Identification of Clerodendrum phlomidis Linn. by GC-MS Analysis and its Acetylcholinesterase Inhibitory Activity}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {April 2022}, pages = {166-171}, type = {Original Article}, chapter = {166}, abstract = {

Background: Agnimantha is an important Ayurvedic medicinal plant mentioned in the text of Acharya charak. Agni means fire and mantha means to rub together to produce fire in ancient times. Objectives: Clerodendrum phlomides Linn. (CP) leaf extracts examined for their phytochemical composition and antioxidant properties, as well as their ability to inhibit acetylcholinesterase (AchE). Materials and Methods: A coarse powder was ground from the leaves after being shade dried at room temperature. Alcoholic and hydroalcoholic extracts of leaf tissues were dried and used to identify phytochemicals by gas chromatography, in vitro radical scavenging assays, and AchE inhibition assays. Results: In the phytochemical screening procedures, fifteen constituents were identified in the alcoholic leaf extract of CP. AchE was inhibited by the extracts in a dose dependent manner as demonstrated by in vitro scavenging tests including FRAP assay (alcoholic 215.20 μg/ml and hydro alcoholic 288.70 μg/ ml) and DPPH radical (alcoholic 167.70 μg/ml and hydro alcoholic 347.19 μg/ml). Inhibition of AchE was observed at 250 μg/ml of CP alcoholic and hydroalcoholic leaf extracts. Conclusion: We demonstrate that CP inhibits AchE activity and is capable of scavenging radicals in vitro, indicating that it could serve as an effective therapy for reversing the cholinergic deficit caused by Neurological diseases.

Key words: Clerodendrum phlomides, Antioxidants, Acetylcholinesterase, Phytochemical identification, GC-MS analysis, Oxidative stress.

}, doi = {10.5530/pres.14.2.24}, author = {Srinivasan Marimuthu and Arun Manohar Gurav and Goli Penchala Prasad} } @article {107, title = {Molecular Identification and Next-Generation Sequence Analysis of Interspecies Genetic Variations among Three Varieties of Datura}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {158-162}, type = {Original Article}, chapter = {158}, abstract = {

Background: Datura is a well-known plant used in Ayurveda. It is a widely growing plant from Solanaceae family, attributed to poisonous and medicinal values. It is used in the treatment of various skin diseases, fever, etc., It is also used for external application and rarely for oral administration. Objectives: The present study aims to compare three different varieties of Datura which include the species of Datura innoxia Mill., Datura metel L. and Datura arborea are used to study molecular marker and phylogenetic analysis. Materials and Methods: Whole-genomic DNA was isolated from the leaves of Datura and the polymerase chain reactions amplification of DNA barcoding markers are rbcl, mat k, and internal transcribed spacer-4 and 5 were analyzed by 0.8\% agarose gel electrophoresis. Results: The DNA barcoding markers and next-generation sequencing are able to identify the interspecies genetic variations among these closely related plant varieties of Datura. Conclusion: The interspecies genetic variations among these closely related three species of D. innoxia, metel, and arborea was closely related with Datura stramonium isolate NN003 chloroplast genome similarity of 98\%, 99\%, and 99\%, respectively.

}, keywords = {Ayurveda, Datura, Internal transcribed spacer 4, Mat k, Medicinal plants, Next-generation sequencing, Ribulose bisphosphate carboxylase/oxygenase form I gene}, doi = {10.4103/pr.pr_101_19}, author = {Goli Penchala Prasad and Goli Penchala Pratap and Srinivasan Marimuthu and Shyam Baboo Prasad and Gajendra Rao and Anupam Kumar Mangal and Naryanam Srikanth} } @article {672, title = {Phytochemical Screening Studies on Melia Orientalis by GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {May 2013}, pages = {216-218}, type = {Original Article }, chapter = {216}, abstract = {

Background:Melia orientalis\ (MO) is an important Ayurvedic medicinal plants. The plant part such as leaves and roots are traditionally used for the treatment of diabetes, edema, traumatic swelling, skin diseases, oligospermia and bleeding disorders.\ Objective:\ To investigate the phytochemical identification of ethanol leaf extract of MO.\ Materials and Methods:\ The fresh leaves of MO (1000g) were collected and shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanol leaf extract obtained was dried and used for phytochemical identification by GC-MS analysis.\ Results:\ The phytochemical screening studies have been carried out and identified ten chemical constituents present in the leaf extract of MO.\ Conclusion:\ Thus, our results show that MO possess important phytocomponents such as phytol, squalene and stigmasterol.

}, keywords = {GC-MS Analysis, Medicinal l Pants, Melia Orientalis, Phytocomponents}, doi = {10.4103/0974-8490.112433}, author = {Srinivasan Marimuthu and Balakrishnan Padmaja and Sudarsan Nair} }