@article {632, title = {The Triterpenoid Fraction from Trichosanthes Dioica Root Exhibits in Vitro Antileishmanial Effect Against Leishmania Donovani Promastigotes}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {April 2013}, pages = { 109-112}, type = {Original Article }, chapter = {109}, abstract = {

Background:Trichosanthes dioica\ Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber found wild throughout the plains of the Indian subcontinent and traditionally used in India for several medicinal purposes.\ Objective:\ The present study was aimed at the evaluation of\ in vitro\ antileishmanial effect of triterpenoid fraction from\ T. dioica\ root (CETD).\ Materials and Methods:\ The antileishmanial activity of CETD was evaluated against\ Leishmania donovani\ (strain MHOM/IN/83/AG83)) promastigotes by\ in vitro\ promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Potassium antimonyl tartrate was used as reference.\ Results:\ Here, CETD markedly inhibited the growth of\ L. donovani\ promastigotes\ in vitro\ in a concentration dependent manner and demonstrated IC\ 50\ value of 18.75 μg/ml. The reference drug potassium antimonyl tartrate exhibited IC\ 50\ of 7.52 μg/ml.\ Conclusion:\ From the present study it can be inferred that the triterpenoid fraction of\ T. dioica\ root exhibited remarkable antileishmanial activity against\ Leishmania donovani\ promastigotes\ in vitro.

}, keywords = {Antileishmanial, Cucurbitacins, Leishmania Donovani, Ppromastigotes, Root, Trichosanthes Dioica}, doi = {10.4103/0974-8490.110540}, author = {Sanjib Bhattacharya and Moulisha Biswas and Pallab K Haldar} } @article {947, title = {Evaluation of in vitro Cytotoxic Effect of Trichosanthes dioica Root}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {January 2011}, pages = {355-358}, type = {O}, chapter = {355}, abstract = {

Background: Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber grown in India and used traditionally for various medicinal purposes. Methods: Present study was aimed to evaluate in vitro cytotoxic effect of dichloromethane (DCTD), methanol (METD), and aqueous (AQTD) extracts of T. dioica root using Allium cepa root meristems by keeping them in different concentrations of each test extract under specific experimental conditions followed by determination of root growth inhibition (root length and number) and mitotic index. Results: All the extracts significantly demonstrated concentration-dependent inhibition of root length and number and reduction in mitotic index, indicating antimitotic activity demonstrating cytotoxicity and genotoxicity. DCTD was found to be the most potent (EC 50 : 2.8 mg/ml), followed by METD and AQTD. Conclusion: The present study therefore, establishes promising in vitro cytotoxic and genotoxic property of T. dioica root against the test system.

}, keywords = {Allium cepa, Antimitotic, Cytotoxic, Root}, doi = {10.4103/0974-8490.75454}, author = {Sanjib Bhattacharya and Pallab K Haldar} }