@article {93, title = {Development and Validation of Veratric Acid in Tabebuia avellanedae using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry-Multiple Reaction Monitoring-Based Assay Coupled with 1,1-Diphenyl-2-Picrylhydrazyl Method}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {107-111}, type = {Original Article}, chapter = {107}, abstract = {

Objective: The aim of the present study is to develop a new simple, precise, and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of veratric acid in Tabebuia avellanedae Linn. Materials and Methods: The quantification of veratric acid was done using collision-induced dissociation multiple reaction monitoring scan of mass spectrometry technique. The developed method was validated according to the International Conference on Harmonization guidelines. The free radical scavenging activity was performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Results: The ion transitions of the precursor to the productions were principally protonated ion [M + H]+ at m/z 182.8 \>139.20, 124.20, and 77.0 for veratric acid. The proposed method was validated for linearity with an excellent correlation coefficient of 0.9978. The intraday and intermediate precisions and repeatability showed the percentage relative standard deviation was \<2\%. The accuracy for the determination of veratric acid was within 82.20\%{\textendash}97.65\%. The limit of detection and limit of quantitation were 0.66 and 2.21 ppm, respectively. The half-maximal inhibitory concentration value for the DPPH radical scavenging activity of ascorbic acid and quality control sample (hydroalcoholic extract of T. avellanedae) was found to be 17.79 and 36.44 μg/ml, respectively. Conclusion: The developed LC-MS/MS method is a simple, rapid, precise, accurate, and it is recommended for efficient assays in routine work. T. avellanedae exhibited strong DPPH radical scavenging activity.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, Method validation, Multiple reaction monitoring, Tabebuia avellanedae, Veratric acid}, doi = {10.4103/pr.pr_77_19}, author = {Puttaswamy Naveen and Harakanahalli Basavegowda Lingaraju and Siddappa Chandrappa and Srikant Anitha and Kodimule Shyam Prasad} } @article {431, title = {Validation of High-Performance Liquid Chromatography Method for Quantification of Costunolide Content in Laurus nobilis}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {422-425}, type = {Original Article}, chapter = {422}, abstract = {

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high-performance liquid chromatography (HPLC). Materials and Methods: The separation was performed on a reversed-phase C18 column (100 {\r A}, 150 mm {\texttimes} 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines. Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r2 \> 0.999). The relative standard deviation (RSD) is less than 1\% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54\%{\textendash}102.62\%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively. Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work.

}, keywords = {Costunolide, Laurus nobilis, Linearity, Precision, Recovery, Validation}, doi = {10.4103/pr.pr_31_18}, author = {Puttaswamy Naveen and Harakanahalli Basavegowda Lingaraju1 and Siddappa Chandrappa1 and Koretekera Shekharappa Priya, and Kodimule Shyam Prasad} }