@article {529, title = {Alpha-amylase inhibitory activity and phytochemical study of Zhumeria majdae Rech. f. and Wendelbo}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {October 2015}, pages = {309-313}, type = {Original Article}, chapter = {309}, abstract = {

Background: Zhumeria majdae (Lamiaceae) is an endemic species growing in the South parts of Iran especially Hormozgan province. The plant is so-called Mohrekhosh locally and widely used for medicinal purposes including stomachache and dysmenorrhea. Objectives: In order to separation and identification of the main flavonoid glycosides of the plant (aerial parts including leaves, stems, flowers, and fruits were used) and evaluation of its alpha-amylase inhibitory (AAI) activity, methanolic extract was prepared and fractionated to botanolic portion. Materials and Methods: Isolation of the main compounds of the butanol extract of the plant have been performed using different column chromatography methods such as high-performance liquid chromatography (C18 column) and Sephadex LH-20 as well. The isolated compounds were identified by Hydrogen-1 nuclear magnetic resonance and Carbon-13 nuclear magnetic resonance spectra and comparison with those reported in previous literature. Moreover, inhibitory activity of the butanolic extract of the plant against alpha-amylase enzyme was examined in different concentrations (15{\textendash}30 mg/mL), where acarbose used as a positive control. Results: Three flavonoid glycosides: Linarin (1), hispidulin- 7-O-(4-O-acetyl-rutinoside) (2), hispidulin-7-O-rutinoside (3) were successfully identified in the extract. The activity of alpha amylase enzyme was dose-dependently suppressed by the butanol extract. The extract exhibited the highest inhibition at 30 mg/mL toward enzyme (77.9 {\textpm} 2.1\%), while acarbose inhibited the enzyme at 20 mg/mL by 73.9 {\textpm} 1.9\%. The inhibitory concentrations of 50\% for the extract and acarbose were calculated at 24.5 {\textpm} 2.1 and 6.6 {\textpm} 3.1 mg/mL, respectively. Conclusion: Z. majdae contains glycosylated flavones and could be a good candidate for anti-diabetic evaluations in animal and clinical trials due to possessing AAI activity.

}, keywords = {Alpha-amylase inhibitor, Hispidulin-7-O-(4-O-acetyl-rutinoside), Hispidulin-7-O-rutinoside, Linarin, Zhumeria majdae.}, doi = {10.4103/0974-8490.158442}, author = {Behnaz Mirshafie and Najmeh Mokhber-Dezfouli and Azadeh Manay and Soodabeh Saeidnia and Yousef Ajani and Ahmad Reza Gohari} }