@article {154, title = {Effect of a Thai Folk Recipe on Phytochemical Screening, Antioxidant Activities, and α-Glucosidase Inhibition by Different Solvent Extracts}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {August 2020}, pages = {225-229}, type = {Original Article}, chapter = {225}, abstract = {

Context: Thai traditional medicine (TTM) has been widely use to treatment of various diseases. Aims: The aims of this study were determined on phytochemical screening, antioxidations, and α-glucosidase inhibition by different solvent extractions. Materials and Methods: The five medicinal plants from a TTM recipe were extracted using aqueous, 50\% ethanol and 95\% ethanol. The phytochemical screening were determined on total phenolic (TPC) and flavonoid (TFC) contents. Their antioxidant activities were tested using by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging assay, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. Glucose transferase mechanism was evaluated using by α-glucosidase inhibitory assay. Results: The results found that the recipe was ingredient with TPC and TFC. The aqueous extract (AE) (47.955 {\textpm} 0.256 mgGE/gExt) was significantly highest amount on TPC while, the ethanolic extract (EE) (1.988 {\textpm} 0.038 mgQE/gExt) was showed significantly highest amount on TFC. The antioxidations, DPPH, and the AE (IC50 = 0.227 {\textpm} 0.005) were significantly more potent on free radical scavenging. ABTS+, AE (IC50 = 0.148 {\textpm} 0.007), Hydro-ethanolic extract (HEE) (IC50 = 0.161 {\textpm} 0.008), and EE (IC50 = 0.151 {\textpm} 0.007) were not different on this method. FRAP, EE (12.681 {\textpm} 0.620 mgTE/gEt) were significantly more potent on cation radical reducing. The α-Glucosidase inhibitory activity, AE (IC50 = 0.021 {\textpm} 0.002 mg/mL) and HEE (IC50 = 0.076 {\textpm} 0.003 mg/mL) were significantly more potent on EE (IC50 = 0.292 {\textpm} 0.010 mg/mL) and Acarbose{\textregistered} (IC50 = 1.05 {\textpm} 0.110 mg/mL). Conclusion: The study confirms traditional use of a Thai folk herbal plants on antioxidation and α-glucosidase inhibition. The recipe was contained with also TPC and TFC might be more potential to antioxidant activities and anti-α-glucoxidase enzyme. Future study, we should be performed to clarify the mechanisms, major active compounds and in vivo.

}, keywords = {Antioxidation, Flavonoids, Phenolic compounds, Thai folk recipe, α-Glucosidase}, doi = {10.4103/pr.pr_100_19}, author = {Anirut Namwong and Pramote Thongkrajai and Ampa Konsue} }