@article {503, title = {Hepatoprotective and Cytotoxic Activities of Abietic Acid from Isodon wightii (Bentham) H. Hara}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {May 2016}, pages = {206-208}, type = {Short Communication}, chapter = {206}, abstract = {

Isodon (Lamiaceae) is a known source of bioactive terpenoids. Diterpenoids isolated from Isodon wightii (Bentham) H. Hara showed antibacterial, antiacetylcholinesterase, antioxidant, anticancer, and anticarcinogenic activities, etc., Hepatoprotective activity of ABA against lipopolysaccharide (LPS) induced liver injury in BALB/c mice was studied. Cytotoxic activity of ABA was tested against cervical cancer cells (HeLa) using MTT assay followed by propidium iodide (PI) staining to identify apoptosis. Histopathological analysis revealed that 1.5 μg/mL LPS induced liver damage was attenuated by ABA in a dose dependent manner. ABA showed cytotoxicity with IC50 value of 176.28 {\textpm} 0.02 μg/mL and PI staining of treated cells showed apoptosis. This study proves that ABA would be a promising natural compound for herbal drug preparation.

}, keywords = {Abietic acid, Apoptosis, BALB/c mice, HeLa, lipopolysaccharide}, doi = {10.4103/0974-8490.182920}, author = {Madhusudhanan Gogul Ramnath and Ramaraj Thirugnanasampandan and Sadasivam Mathusalini and Palathurai Subramanium Mohan} } @article {572, title = {Chemical Composition Analysis, Antioxidant and Antibacterial Activity Evaluation of Essential oil of Atalantia monophylla Correa}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {s52-s56}, type = {Original Article}, chapter = {s52}, abstract = {

Background: Atalantia monophylla Correa. a small tree belongs to the family Rutaceae. It is distributed throughout India and in Tamil Nadu the species is commonly seen in foothills of dry vegetation. Objective: The aim was to hydrodistillate and analyze the chemical composition of essential oil from the fresh leaves of A. monophylla Correa. collected in two different seasons (December, 2013 and May, 2014) and to evaluate antioxidant and antibacterial activities of isolated essential oil. Materials and Methods: Chemical composition of isolated essential oil was analyzed by gas chromatography, gas chromatography coupled with mass spectrometry. Antioxidant activity of oil was assessed using five different antioxidant test systems. Antibacterial activity of oil was tested against six pathogenic bacteria by broth dilution method. Results: Essential oil obtained from the leaves collected during May, 2014 had shown more compounds. Antioxidant activity of oil was moderate when compared with positive control. Minimum inhibitory concentration value of oil was ranges between 139.32 {\textpm} 0.001 and 541.11 {\textpm} 0.003 {\textmu}g/mL against all the tested bacteria. Conclusion: Result clearly indicates essential oil collected during May, 2014 showed more bioactive compounds.

}, keywords = {Antibacterial activity, Antioxidant, Atalantia monophylla, Essential oil, Gas chromatography coupled with mass spectrometry}, doi = {10.4103/0974-8490.152009}, author = {Ramaraj Thirugnanasampandan and Ramya Gunasekar and Madhusudhanan Gogulramnath} }