@article {484, title = {Characterization of Anticancer Principles of Celosia argentea (Amaranthaceae)}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {February 2016}, pages = {97-104}, type = {Original Article}, chapter = {97}, abstract = {

Background: An Indian origin, Celosia argentea is a weed growing during rainy season traditionally claimed for treating several ailments. Early researches on C. argentea were focused on the anti-cancer screening of seeds, with few reports on aerial parts. Objective: To isolate and characterize bioactive compounds of aerial parts of C. argentea and evaluate their anticancer potential. Materials and Methods: The methanolic aerial part extract was fractionated on column chromatography using chloroform: methanol mixture. The fractions; 80:20 and 95:5 were purified on MCI-HP20 HPLC column. Chromatographically pure compounds were pooled, concentrated and characterized spectroscopically. The compounds were further screened for antioxidant and cytotoxic potential. Results: Isolated compounds were confirmed as: (1) Luteolin-7-O-glucoside and (2) phenolic, 1-(4-hydroxy- 2-methoxybenzofuran-5-yl)-3-phenylpropane-1,3-dione. Both exhibited significant antioxidant potential with IC50 values of 20.80 and 21.30 μg/ml for 2,2-diphenyl-1-picrylhydrazyl assay (***P \< 0.001) and significant Trolox equivalent antioxidant capacity (TEAC) values for 2,2{\textquoteright}-azino-bis(3- ethylbenzthiazoline-6-sulphonic acid) (*P \< 0.05) and ferric reducing antioxidant potential assay (****P \< 0.0001). In 3(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazoliumbromide assay, Compound 1 and 2 showed potent cytotoxicity against SiHa, HCT, MCF-7 cancer cell lines at 20 μg/ml (****P \< 0.0001) and 18 μg/ml (**P \< 0.01), respectively, without affecting the normal Vero cells. Both compounds enabled maximum reduction in cell viability at 50 μg/ml against HT-29 (***P \< 0.001) and MCF-7 cell lines (**P \< 0.01) in try pan blue viability assay. Apoptosis occurred at concentrations of 47.33 {\textpm} 0.8 μg/ml and 56.28 {\textpm} 1.2 μg/ml for Compound 1 and 35.15 {\textpm} 0.4 μg/ml and 28.05 {\textpm} 0.3 μg/ml for Compound 2 for HT-29 and MCF-7 respectively. Conclusion: A novel anticancer phenolic compound; (1-(4-hydroxy-2-methoxybenzofuran-5-yl)-3-phenylpropane- 1,3-dione), isolated from aerial parts of C. argentea was a valuable finding of the research.

}, keywords = {2, 2-diphenyl-1-picrylhydrazylDPPH, 3(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyl-tetrazoliumbromideMTT, anti-oxidant, anticancer, Celosia argentea}, doi = {10.4103/0974-8490.172659}, author = {Rukhsana A. Rub and Manohar J. Pati and Areej A. Siddiqui and Alpana S. Moghe and Nasreen N. Shaikh} } @article {509, title = {Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular Assays}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {231-237}, type = {Original Article}, chapter = {231}, abstract = {

Background: Molecules stimulating regeneration and proliferation of cells are of significance in combating ailments caused due to tissue injury, inflammation, and degenerative disorders. Moringa oleifera is one of the most valued food plants having the profile of important nutrients and impressive range of medicinal uses. Objective: To evaluate the potential of M. oleifera aqueous leaf and flower extracts to promote the proliferation of cells and explore their effect on cancer cell lines for assessment of safety. Materials and Methods: Aqueous leaf and flower extracts of M. oleifera were investigated for effect on rat-derived primary fibroblast, mesenchymal stem cells (MSCs), and cancer cell lines using cell proliferation assay. They were also tested and compared for wound healing, angiogenesis, and hepatoprotective effect using in vitro assays. Results: Statistically significant increase in the proliferation of primary rat fibroblast, MSCs, and angiogenesis was observed after treatment with aqueous flower extract. The aqueous leaf extract determined a comparatively moderate increment in the proliferation of MSCs and angiogenesis. It however showed prominent cytotoxicity to cancer cell lines and a significant hepatoprotective effect. Conclusion: A very clear difference in response of the two extracts to different types of cells was detected in this study. The aqueous flower extract exhibited a higher potential to stimulate cell proliferation while not exerting the same effect on cancer cell lines. The leaf extract on the other hand, had a prominent antitumor and hepatoptotective effects.

}, keywords = {Aqueous, cytotoxicity, Fibroblast, in vitro, Viability}, doi = {10.4103/0974-8490.188877}, author = {Evangeline E. Fernandes and Anubha V. Pulwale and Gauri A. Patil and Alpana S. Moghe} }