@article {545, title = {Antioxidant and Antiacetylcholinesterase Activity of Teucrium Hyrcanicum,}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {s15-s19}, type = {Original Article }, chapter = {s15}, abstract = {

Background:Teucrium hyrcanicum\ belonging to the Lamiaceae family is a native plant in Iran; it is called Maryam nokhodi-e-jangali in Farsi.\ Objective:\ The aim of this study is to evaluate acetylcholinesterase inhibition (AChEI), antioxidant activity and flavonoids content of\ T.hyrcanicum\ methanol extract.\ MaterialsandMethods:\ The air-dried and the ground aerial parts of\ T. hyrcanicum\ were extracted by percolation method with methanol. Antioxidant activity of the extract was investigated by using 2,2{\textquoteright}-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay (FRAP) methods. In addition, AChEI and flavonoid content of\ T. hyrcanicum\ methanol extract were measured.\ Results:\ The results showed that total flavonoid content of\ T. hyrcanicum\ in reference to the standard curve for quercetin was 20.70 {\textpm} 0.05 mg quercetin equivalents/g of extract. In the FRAP method, the antioxidant activity of\ T. hyrcanicum\ extract and butyl hydroxyanisole (BHA) (as a positive control) were 657.5 {\textpm} 0.04 and 880 {\textpm} 0.06 mmol Fe II/1 g dried extract. According to results of DPPH assay, half maximal inhibitory concentration (IC50\ ) value for DPPH radical-scavenging activities of\ T. hyrcanicum\ methanol extract, vitamin E and BHA were 74.6, 14.12 and 7.8 μg/mL, respectively. IC\ 50\ value for AChEI of\ T. hyrcanicum\ and donepezil as a positive control were 2.12 mg/mL and 0.013 mg/mL.\ Conclusion:\ The results of the present study showed\ T. hyrcanicum\ is a natural antioxidant that the flavonoid content can be responsible for extract effects.

}, keywords = {Aantioxidant Activity, Acetylcholinesterase Inhibitory, Flavonoid Contents, Teucrium Hyrcanicum}, doi = {10.4103/0974-8490.157993 }, author = {Fereshteh Golfakhrabadi and Fatemeh Yousefbeyk and Tahmineh Mirnezami and Pedram Laghaei and Mannan Hajimahmoodi and Mahnaz Khanavi} } @article {427, title = {In vitro Antioxidant and Free Radical Scavenging Activity of Four Alkanna Species Growing in Iran}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {December 2014}, pages = {100-104}, type = {Original Article}, chapter = {100}, abstract = {

Background: Medicinal plants are recognized as sources of natural antioxidants that can protect biological system from oxidative stress. Objective: In this study, the antioxidant activities of four species of the Alkanna genus (Alkanna bracteosa, Alkanna frigida, Alkanna orientalis and Alkanna tricophila) were evaluated. Materials and Methods: The dried roots of plant samples (100 g) were extracted separately by percolation method with MeOH/H2O (80/20) and four fractions were provided respectively with hexane, chloroform, ethyl acetate, butanol and water. Antioxidant activities were investigated by using 1,1-diphenyl-2-picrylhydrazyl, ferric thiocyanate and thiobarbituric acid methods and compared with quercetin (as positive control). Results: The results indicated that the butanol fractions of four species had the highest antioxidant activity and radical scavenging activity compared with the other fractions studied (P \< 0.05). The 50\% effective concentration (half -\ effective doses) values of butanol fractions are less than quercetin and other fractions, so, these fractions showed potent antioxidant activity. This indicated that the active compounds in the root parts of Alkanna species dissolved in the butanol. All the fractions of four species of Alkanna had antioxidant activity, while, there were no significant differences (P \> 0.05) between the total antioxidant activities of same fractions. The total antioxidant activity values of Alkanna fractions in a descending order are as follows: Butanol fraction \> ethyl acetate fraction \> total extract \> hexane fraction \> chloroform fraction \> aqueous fraction. Conclusion: The antioxidant activities of butanol fractions of samples were higher than quercetin. This may be because most of the active compounds of Alkanna species dissolved in the butanol.

}, keywords = {1, 1-diphenyl-2-picrylhydrazyl, Alkanna, Antioxidant activity, Ferric thiocyanate, Thiobarbituric acid.}, doi = {10.4103/0974-8490.147218}, author = {Iraj Salimikia and Ali Reza Yazdinezhad and Fereshteh Golfakhrabadi and Hamid Reza Monsef Esfahani} }