@article {1141, title = {Total Polyphenolic Content, Antioxidant Activity and Chromatographic Profiling of Extracts of Ardissia solanacea}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {356-362}, type = {Original Article }, chapter = {356}, abstract = {

Background: The current study focuses on the preliminary phytochemical analysis, antioxidant potential and identification of phytochemicals in Ardissia solanacea leaves extract using chromatography and HR-MS. Materials and Methods: The occurrence of phytoconstituents in different extracts of Ardissia solanacea were assessed by standard procedures. In vitro antioxidant potential was determined by Hydrogen peroxide and DPPH method. The extract was fractionated by Flash Chromatography. The fractions were analysed using HPTLC and further analyzed by HR-MS method to detect and characterize the active phytoconstituents present in it. Results: The highest concentration of flavonoids (371.91{\textpm} 0.167 mg/g of Quercetin Equivalent), phenolics (10.138{\textpm}0.010 mg/g of Gallic acid Equivalent) and tannins (148.23{\textpm}0.510 mg/g of Tannic acid Equivalent) was observed in the ethanol extract. The antioxidant activity by DPPH and H2O2 assay demonstrated that the ethanolic extract (AsEt) showed highest anti-oxidant potential (74.21\% and 83.16\% respectively). The ASEt was fractionated by using Flash chromatography. As a result, Out of 11 fractions, one fraction namely AsEt3 was selected further based on HPTLC results. The HR-MS analysis of ASEt3 indicated presence of thirteen compounds wherein bergapten was predominantly identified. Conclusion: It was revealed that the leaves of Ardissia solanacea possesses high antioxidant potential. The known phytoconstituents namely quercetin and taxifolin along with one unknown compound were identified through the HR-MS study. Using mass library data, we report herein 2 flavonoids and 1 phenolic in the AsEt extract.

}, keywords = {Antioxidant activity, Ardissia solanacea, Flash chromatography, HR-MS analysis.}, doi = {10.5530/pres.15.2.038}, author = {Jyotsna Chopade and Asha Thomas and Sohan Chitlange} } @article {405, title = {Evaluation of anti-inflammatory and antimicrobial activity of AHPL/AYCAP/0413 capsule}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {July 2017}, pages = {273-276}, type = {Original Article}, chapter = {273}, abstract = {

Background: Conventional therapeutic agents used for treatment of Acne are associated with various adverse effects necessitating development of safe and effective alternative therapeutic agents. In this context, a polyherbal formulation AHPL/AYCAP/0413 was developed for treatment of Acne. Objectives: To evaluate Anti-inflammatory and antimicrobial activity of AHPL/AYCAP/0413. Material and Methods: 1) Anti-inflammatory activity: Anti-inflammatory activity of AHPL/AYCAP/0413 in comparison with Diclofenac was assessed in carrageenan induced rat Paw edema model. 2) Anti-microbial activity for P. acne: Propionibacterium acnes were incubated under anaerobic conditions. Aliquots of molten BHI with glucose agar were used as the agar base. Formulation and clindamycin (10 μg/ml) were introduced in to the Agar wells randomly. 3) Anti-microbial activity for Staphylococcus epidermidis and Staphylococcus aureus: Staphylococcus epidermidis and Staphylococcus aureus were incubated under aerobic conditions at 37{\textdegree}C. TSB with glucose agar was used as the agar base. 0.5ml of formulation and clindamycin (10 μg/ml) were introduced in to the wells randomly. The antibacterial activity was evaluated by measuring zones of inhibition (in mm). Result: Significant reduction in rat paw edema (51\% inhibition) was observed with formulation AHPL/AYCAP/0413 which was also comparable to that of Diclofenac (58\% inhibition). Zone of inhibition for formulation was 18.33 mm, 19.20 mm and 26.30 mm for P. acnes, S. epidermidis and S. aureus respectively. This activity was also comparable to that of Clindamycin. Conclusion: AHPL/AYCAP/0413 capsule possesses significant Anti-inflammatory and Anti-microbial activities which further justifies its role in the management of Acne vulgaris.

}, keywords = {Acne vulgaris, AHPL/AYCAP/0413 capsule, Alternative system of medicine, Herbal}, doi = {10.4103/0974-8490.210328}, author = {Sanjay Nipanikar and Sohan Chitlange and Dheeraj Nagore} }