@article {91, title = {Inhibitory Actions of Lupinifolin Isolated from Derris reticulata Stem against Carbohydrate-Digesting Enzymes}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {May 2020}, pages = {102-106}, type = {Original Article}, chapter = {102}, abstract = {

Background: Postprandial hyperglycemia is linked with the development of diabetic complications. Inhibition against the carbohydrate-digesting enzymes, specifically pancreatic α-amylase and intestinal α-glucosidase, delays carbohydrate digestion and subsides postprandial plasma glucose (PPG) levels. Objectives: This study aimed to examine the effects of lupinifolin, purified from Derris reticulata stem, a medicinal plant traditionally used for the treatment of diabetes mellitus, on in vitro α-amylase and α-glucosidase enzyme activities and enzyme kinetics. Subjects and Methods: The identification of the isolated phytochemical was performed using nuclear magnetic resonance{\textendash}mass spectrometry (NMR-MS) spectrometry. In vitro pancreatic α-amylase and α-glucosidase activities and enzyme kinetics were determined using the enzymatic colorimetric methods. Results: The purified phytochemical was identified as lupinifolin from the NMR-MS spectrometry. The isolated lupinifolin produced a significant α-amylase and α-glucosidase inhibitory actions with the median inhibitory concentrations of 3.43 {\textpm} 0.77 and 56.29 {\textpm} 11.64 μg/mL, respectively. The study of enzyme kinetics showed that lupinifolin exhibited the mode of non-competitive and mixed enzyme inhibitions against α-amylase and α-glucosidase, respectively. Conclusion: The potent α-amylase and α-glucosidase inhibitory actions of lupinifolin derived from D. reticulata stem evidently suggest its potential use as an alternative for the control of PPG.

}, keywords = {Derris reticulate, Diabetes mellitus, Lupinifolin, α-amylase, α-glucosidase}, doi = {10.4103/pr.pr_117_19}, author = {Pawitra Pulbutr and Pichnatcha Nantana and Surabot Suksabai and Chawannuch Mudjupa and Rattazart Denchai and Sakulrat Rattanakiat and Taweesak Dhammaraj} }