@article {732, title = {Antifouling Alkaloids from Crinum augustum (Amaryllidaceae)}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {43-52}, type = {Original Article}, chapter = {43}, abstract = {

Fractionation and purification of the ethanolic extract of the bulbs of Crinum augustum Rox. (Amaryllidaceae) cultivated in Egypt yielded five alkaloids 6-methoxy-crinamine (1), crinamine (2), buphanisine (3), ungeremine (4), and hippadine (5); two fatty acid derivatives: myristic acid ethyl ester (6) and palmitic acid ethyl ester (7); four terpenoidal and steroidal compounds: ursolic acid (8), \%-sitosterol-O- \% glucopyranoside (9) and mixture of \%- sitosterol (10) and stigmasterol (11). The structures of all compounds were determined by interpretation of their spectroscopic data; 1D (1H and 13C), 2D (HSQC, COSY, DQF, NOE and HMBC) NMR; MS and UV analyses. The compounds (1-4) and (6-8) were tested towards biofouling activity using larvae of barnacle Balance amphitrie. Significant activities of 1, 2 and 3 with EC50 1.8, 1.2 and 0.75 :g/mL respectively, were observed.

}, keywords = {Amaryllidaceous, Anti-fouling, Crinum augustum, Natural Products}, author = {John Refaat and Ahmed Abdel-Lateff and Mohamed S. Kamel and Ahmed A. Ali and Mahmoud A. Ramadan and Tatsufumi Okino and Yasuyuki Nogata} }